Jilin Academy of Chinese Medicine and Material Medica Science, Changchun 130012, PR China.
J Ethnopharmacol. 2013 Jul 30;148(3):946-50. doi: 10.1016/j.jep.2013.05.049. Epub 2013 Jun 5.
The root of Panax ginseng C.A. Mey has various beneficial pharmacological effects. The present study aimed to evaluate the analgesic activities of glycoproteins from the root of Panax ginseng C.A. Mey in mice.
Glycoproteins were isolated and purified from the root of Panax ginseng C.A. Mey. Physicochemical properties and molecular mass were determined by chemical assay and HPLC. Acetic acid-induced writhing and hot-plate tests were employed to study the analgesic effect of glycoproteins and compared with that of aspirin or morphine. The locomotor activity was tested in mice by using actophometer.
Four glycoproteins were obtained. The glycoproteins which protein content was the highest (73.04%) displayed dose-dependent analgesic effect. In writhing test, the glycoproteins significantly inhibited writhes (P<0.001) at the dose of 20 mg/kg by intraperitoneal injection. In hot-plate test, only at the dose of 20 mg/kg prolong the hot-plate latency (P<0.05, at 30 min). In the locomotor activity test, the glycoproteins were significant decrease of motility counts at the dose of 20 and 40 mg/kg.
These findings collectively indicate that the glycoproteins from the root of Panax ginseng C.A. Mey exhibited significant analgesic activities and the proteins were the active site, providing evidence for its pharmacal use.
人参的根具有各种有益的药理作用。本研究旨在评估人参根糖蛋白在小鼠中的镇痛活性。
糖蛋白从人参根中分离和纯化。通过化学分析和 HPLC 测定理化性质和分子量。采用醋酸诱导扭体和热板试验研究糖蛋白的镇痛作用，并与阿司匹林或吗啡进行比较。使用活动计测试小鼠的运动活性。
得到了 4 种糖蛋白。糖蛋白的蛋白质含量最高（73.04%），具有剂量依赖性的镇痛作用。在扭体试验中，糖蛋白在 20mg/kg 的剂量下通过腹腔注射显著抑制扭体（P<0.001）。在热板试验中，只有在 20mg/kg 的剂量下才能延长热板潜伏期（P<0.05，在 30 分钟时）。在运动活性试验中，糖蛋白在 20 和 40mg/kg 的剂量下显著减少运动计数。
这些发现表明，人参根的糖蛋白具有显著的镇痛活性，蛋白质是活性部位，为其药用提供了证据。
