Department of Microbiology, College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Meat Sci. 2013 Oct;95(2):381-8. doi: 10.1016/j.meatsci.2013.04.051. Epub 2013 May 1.
To assess the presences of Escherichia coli, its serogroups, virulence factors and antibiotic resistance properties in ruminant's meat, a total of 820 raw meat samples were collected and then evaluated using culture, PCR and disk diffusion methods. Totally, 238 (29.02%) samples were positive for presence of Escherichia coli. All of the isolates had more than one virulence gene including Stx1, Stx2, eaeA and ehly. All investigated serogroups were found in beef and sheep and all except O145, O121 and O128 were found in goat. The O91, O113, O111, O103, O26 and O157 serogroups were found in camel. Totally, aadA1-blaSHV combination was the most predominant antibiotic resistance gene. The highest resistance of STEC strains was seen against penicillin while resistance to nitrofurantoin and ciprofloxacin was minimal. These findings showed that health care and meat inspection should be reconsidered in Iranian slaughterhouses and butchers.
为了评估食源性大肠杆菌及其血清型、毒力因子和抗生素耐药性在反刍动物肉类中的存在情况，共采集了 820 份生肉样本，然后使用培养、PCR 和药敏扩散方法进行评估。共有 238 份（29.02%）样本呈大肠杆菌阳性。所有分离株均携带一种以上的毒力基因，包括 Stx1、Stx2、eaeA 和 ehly。所有研究的血清型均在牛肉和羊肉中发现，除 O145、O121 和 O128 外，在山羊中也发现了所有血清型。O91、O113、O111、O103、O26 和 O157 血清型在骆驼中发现。总的来说，aadA1-blaSHV 组合是最主要的抗生素耐药基因。STEC 菌株对青霉素的耐药率最高，而对呋喃妥因和环丙沙星的耐药率最低。这些发现表明，伊朗屠宰场和肉铺应重新考虑卫生保健和肉类检查。
