Elbayomi Smaher M, Wang Haili, Tamer Tamer M, You Yezi
Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China.
Polymer Materials Research Department, Advanced Technologies, and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria P.O. Box 21934, Egypt.
Polymers (Basel). 2021 Aug 2;13(15):2575. doi: 10.3390/polym13152575.
The preparation of bioactive polymeric molecules requires the attention of scientists as it has a potential function in biomedical applications. In the current study, functional substitution of alginate with a benzoyl group was prepared via coupling its hydroxyl group with benzoyl chloride. Fourier transform infrared spectroscopy indicated the characteristic peaks of aromatic C=C in alginate derivative at 1431 cm. HNMR analysis demonstrated the aromatic protons at 7.5 ppm assigned to benzoyl groups attached to alginate hydroxyl groups. Wetting analysis showed a decrease in hydrophilicity in the new alginate derivative. Differential scanning calorimetry and thermal gravimetric analysis showed that the designed aromatic alginate derivative demonstrated higher thermo-stability than alginates. The aromatic alginate derivative displayed high anti-inflammatory properties compared to alginate. Finally, the in vitro antioxidant evaluation of the aromatic alginate derivative showed a significant increase in free radical scavenging activity compared to neat alginate against DPPH (2,2-diphenyll-picrylhydrazyl) and ABTS free radicals. The obtained results proposed that the new alginate derivative could be employed for gene and drug delivery applications.
生物活性聚合物分子的制备需要科学家们的关注，因为它在生物医学应用中具有潜在功能。在当前的研究中，通过将藻酸盐的羟基与苯甲酰氯偶联，制备了具有苯甲酰基的藻酸盐功能取代物。傅里叶变换红外光谱表明，藻酸盐衍生物中芳香族C=C的特征峰位于1431 cm处。核磁共振氢谱分析表明，在7.5 ppm处的芳香族质子归属于连接在藻酸盐羟基上的苯甲酰基。润湿性分析表明，新型藻酸盐衍生物的亲水性降低。差示扫描量热法和热重分析表明，所设计的芳香族藻酸盐衍生物比藻酸盐具有更高的热稳定性。与藻酸盐相比，芳香族藻酸盐衍生物表现出高抗炎特性。最后，芳香族藻酸盐衍生物的体外抗氧化评估表明，与纯藻酸盐相比，其对DPPH（2,2-二苯基-1-苦基肼）和ABTS自由基的自由基清除活性显著增加。所得结果表明，新型藻酸盐衍生物可用于基因和药物递送应用。
