Department of Biology, Plant Biotechnology, ETH Zurich, Zurich, Switzerland.
J Proteomics. 2013 Nov 20;93:5-19. doi: 10.1016/j.jprot.2013.05.036. Epub 2013 Jun 6.
In the last decade proteomics studies have gained increasing importance in plant research. The development of proteomics techniques allowing increased proteome coverage and quantitative measurements of proteins have been particularly instrumental to characterize proteomes and their modulation during plant development, biotic and abiotic stresses. Despite important advances, plant proteome analysis, including those of model plant species, remain constrained by limitations inherent to proteomics techniques and data interpretation. Here we review the approaches and achievements of proteomics with model plant and crop species (i.e. Arabidopsis and rice) and discuss the current limitations of crop proteomics. We anticipate future directions that could advance the contribution of plant proteomics to crop improvement.
在过去的十年中,蛋白质组学研究在植物研究中变得越来越重要。蛋白质组学技术的发展使得对蛋白质的全谱覆盖和定量测量成为可能,这对于描述植物发育过程中的蛋白质组及其调控、生物和非生物胁迫具有重要作用。尽管取得了重要进展,但包括模式植物在内的植物蛋白质组分析仍然受到蛋白质组学技术和数据解释固有的限制。本文综述了模式植物和作物(如拟南芥和水稻)的蛋白质组学方法和成就,并讨论了作物蛋白质组学的当前局限性。我们预计未来的发展方向将推动植物蛋白质组学对作物改良的贡献。