Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, Germany.
Prog Lipid Res. 2013 Oct;52(4):465-87. doi: 10.1016/j.plipres.2013.05.003. Epub 2013 Jun 5.
This review highlights technological developments in magnetic resonance imaging (MRI), which are creating opportunities for the three dimensional visualization and quantification of lipids in plant materials. A major feature of MRI is that it is a non-invasive platform, and thus can be used for the analysis of living organisms. An overview of the theoretical aspects of MRI is provided, followed by a description of the various analytical modes available, and an explanation of how MRI can be applied to plant samples and what it can achieve. Various lipid maps and three dimensional models of seeds and fruits are included to demonstrate the potential of MRI and to exemplify recent cutting-edge advances in the field. The importance and prospects of the imaging of lipids in living plants, as well as the integration of lipid imaging with other emerging techniques, are outlined to provide impetus for future plant lipid research.
本文重点介绍磁共振成像(MRI)技术的发展,这些发展为植物材料中脂质的三维可视化和定量分析创造了机会。MRI 的一个主要特点是它是一种非侵入性平台,因此可用于分析活体生物。本文首先概述了 MRI 的理论方面,接着介绍了各种可用的分析模式,并解释了如何将 MRI 应用于植物样本以及它可以实现什么。本文还包括各种种子和果实的脂质图谱和三维模型,以展示 MRI 的潜力,并举例说明该领域最近的前沿进展。本文最后概述了在活体植物中对脂质进行成像的重要性和前景,以及将脂质成像与其他新兴技术相结合,为未来的植物脂质研究提供动力。