Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang, 438000, China.
Sci Rep. 2018 Apr 26;8(1):6560. doi: 10.1038/s41598-018-24812-2.
Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details might have been lost in previous studies. In the present work, the configuration of LDs in seeds was obtained by confocal imaging combined with 3D reconstruction technology in Brassica napus. The size and shape of LDs, LD numbers, cell interval spaces and cell size were observed and compared at 3D levels in the seeds of different materials with high and low oil content. It was also revealed that different cells located in the same tissue exhibited various oil contents according to the construction at the 3D level, which was not previously reported in B. napus. The present work provides a new way to understand the differential in cell populations and enhance the seed oil content at the single cell level within seeds.
油菜籽是全球最重要、最广泛种植的粮油作物之一。中性脂质以油滴 (LD) 的形式储存，作为发芽和随后幼苗生长的燃料。以前的研究中，种子中大部分 LD 的检测仍处于 2D 水平，一些细节可能已经丢失。在本工作中，通过共聚焦成像结合 3D 重建技术，获得了甘蓝型油菜种子中 LD 的形态。在不同高油和低油含量的材料种子中，在 3D 水平上观察和比较了 LD 的大小和形状、LD 数量、细胞间隔空间和细胞大小。结果还表明，根据 3D 水平的结构，同一组织中不同的细胞表现出不同的含油量，这在甘蓝型油菜中以前没有报道过。本工作为在单细胞水平上理解细胞群体的差异和提高种子含油量提供了一种新方法。
