Université d'Orléans-CNRS, Institut de Chimie Organique et Analytique UMR 7311, BP 6759 rue de Chartres, 45067, Orléans cedex 2, France.
Anal Bioanal Chem. 2014 Feb;406(4):957-69. doi: 10.1007/s00216-013-7017-8. Epub 2013 Jun 8.
Countercurrent chromatography (CCC) is an attractive separation method because the analytes are partitioned between two immiscible liquid phases avoiding problems related to solid stationary phase. In recent years, this technique has made great progress in separation power and detection potential. This review describes coupling strategies involving high speed CCC (HSCCC) or centrifugal partition chromatography (CPC). It includes on-line extraction-isolation, hyphenation with mass spectrometry (MS) and nuclear magnetic resonance (NMR) detectors, multidimensional CCC (MDCCC), two-dimensional CCC (2D-CCC), on-line coupling with liquid chromatography (LC), and biological tests, and innovative off-line developments. The basic principles of each method are presented and applications are summarized.
逆流色谱（CCC）是一种很有吸引力的分离方法，因为分析物在两相之间分配，避免了与固定相有关的问题。近年来，该技术在分离能力和检测潜力方面取得了很大进展。本文综述了涉及高速逆流色谱（HSCCC）或离心分配色谱（CPC）的偶联策略。它包括在线提取-分离、与质谱（MS）和核磁共振（NMR）检测器的联用、多维 CCC（MDCCC）、二维 CCC（2D-CCC）、与液相色谱（LC）的在线偶联以及生物测试和创新的离线发展。介绍了每种方法的基本原理，并总结了应用情况。
