Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro, 00185 Roma, Italy.
Molecules. 2018 Nov 27;23(12):3091. doi: 10.3390/molecules23123091.
Nowadays, there is an increasing attention for nutraceuticals and, in general, bioactive compounds naturally present in food. Indeed, the possibility of preserving human health and preventing disease (e.g., cardiovascular diseases, cancer etc.) by the intake of healthy food is attractive for both consumers and food industries. In turn, research in this field was also prompted significantly, with the aim of characterizing these bioactive compounds and ascribe to them a specific activity. The bioactive compounds can belong to several chemical classes. However, their chemical diversity and presence in complex matrices, such as food, make it challenging both their isolation and characterization. To tackle this issue, efficient separation systems are needed, which are mainly based on chromatography. In this context, this mini-review aims to provide the reader with an overview of the most relevant and recent approaches for the separation of the most common bioactive compounds in food, in particular polyphenols, phenols, carotenoids, and peptides, by liquid chromatography approaches.
如今，人们越来越关注营养保健品，以及一般存在于食物中的生物活性化合物。事实上，通过摄入健康食品来保持人类健康和预防疾病（如心血管疾病、癌症等）的可能性对消费者和食品行业都具有吸引力。反过来，这一领域的研究也得到了显著推动，目的是对这些生物活性化合物进行特征描述，并赋予它们特定的活性。生物活性化合物可以属于几种化学类别。然而，它们的化学多样性和在复杂基质（如食物）中的存在，使得它们的分离和鉴定具有挑战性。为了解决这个问题，需要高效的分离系统，主要基于色谱法。在这方面，本篇迷你综述旨在为读者提供关于通过液相色谱法分离食物中最常见生物活性化合物（特别是多酚、酚类、类胡萝卜素和肽）的最相关和最新方法的概述。
