Özben Hakan, Eralp Levent, Baysal Gökhan, Cort Ayşegül, Sarkalkan Nazli, Özben Tomris
Department of Hand Surgery and Microsurgery, University Hospital, University of Modena and Reggio Emilia, Modena, Italy.
Acta Orthop Traumatol Turc. 2013;47(3):184-92. doi: 10.3944/aott.2013.2828.
Despite wide resection and systemic chemotherapy, bone tumors may present with local recurrences, metastases and pathological fractures. Application of bone cement containing antineoplastic drug to fill the defect after resection of metastatic lesions and to support implants has been suggested to prevent local tumor growth and implant failures. In this study, we aimed to demonstrate the effects of the addition of cisplatin which is a widely used antineoplastic drug for osteosarcoma, on the mechanical properties of bone cement, and to evaluate the cytotoxic effects of eluted cisplatin on Saos-2 cell culture.
Two cement samples were prepared by mixing 100 mg and 300 mg of cisplatin powder with 40 g cement powder. The bone cement of the control group did not contain cisplatin. Mechanical analyses included 4-point bending, compression and shear testing. For cytotoxicity analysis, samples were incubated in Dulbecco's Modified Eagle's medium for 15 days. Mediums were applied to Saos-2 cell culture and cell viability was measured. Surface analyses were performed by scanning electron microscope (SEM).
The addition of cisplatin did not alter the mechanical properties of bone cement. It was observed that the eluted cisplatin had cytotoxic effects on Saos-2 cells. SEM analyses demonstrated cisplatin granules on the surface of cement samples.
Cisplatin maintains its cytotoxic property when released from bone cement without compromising the mechanical stability. Application of cisplatin loaded bone cement may help local control of tumor growth. We believe that our study will shed light on to these new practices for the treatment of bone cancers and will encourage future studies.
尽管进行了广泛切除和全身化疗，骨肿瘤仍可能出现局部复发、转移和病理性骨折。有人建议应用含抗肿瘤药物的骨水泥填充转移性病变切除后的缺损并支撑植入物，以防止局部肿瘤生长和植入物失败。在本研究中，我们旨在证明添加顺铂（一种广泛用于骨肉瘤的抗肿瘤药物）对骨水泥力学性能的影响，并评估洗脱的顺铂对Saos-2细胞培养的细胞毒性作用。
通过将100mg和300mg顺铂粉末与40g骨水泥粉末混合制备两个骨水泥样品。对照组的骨水泥不含顺铂。力学分析包括四点弯曲、压缩和剪切测试。对于细胞毒性分析，将样品在杜氏改良 Eagle 培养基中孵育15天。将培养基应用于Saos-2细胞培养并测量细胞活力。通过扫描电子显微镜（SEM）进行表面分析。
顺铂的添加未改变骨水泥的力学性能。观察到洗脱的顺铂对Saos-2细胞具有细胞毒性作用。SEM分析显示骨水泥样品表面有顺铂颗粒。
顺铂从骨水泥中释放时保持其细胞毒性特性，而不会损害机械稳定性。应用载有顺铂的骨水泥可能有助于局部控制肿瘤生长。我们相信我们的研究将为这些骨癌治疗的新方法提供启示，并鼓励未来的研究。
