Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China.
ACS Appl Mater Interfaces. 2013 Jul 10;5(13):5976-85. doi: 10.1021/am400249n. Epub 2013 Jun 24.
Stem cells contribute to physiological processes such as postischemic neovascularization and vascular re-endothelialization, which help regenerate myocardial defects or repair vascular injury. However, therapeutic efficacy of stem cell transplantation is often limited by inefficient homing of systemically administered cells, which results in a low number of cells accumulating at sites of pathology. In this study, anti-CD34 antibody-coated magnetic nanoparticles (Fe3O4@PEG-CD34) are shown to have high affinity to stem cells. The results of hemolysis rate and activated partial thromboplastin time (APTT) tests indicate that such nanoparticle may be used safely in the blood system. In vitro studies showed that a nanoparticle concentration of 100 μg/mL gives rise to a significant increase in cell retention using an applicable permanent magnet, exerting minimal negative effect on cell viability and migration. Subsequent in vivo studies indicate that nanopartical can specifically bind stem cells with good magnetic response. Anti-CD34 antibody coated magnetic nanoparticle may be used to help deliver stem cells to a lesion site in the body for better treatment.
干细胞有助于生理过程，如缺血后新生血管形成和血管再内皮化，有助于再生心肌缺陷或修复血管损伤。然而，干细胞移植的治疗效果往往受到系统给予的细胞归巢效率低下的限制，导致在病理部位积累的细胞数量较少。在这项研究中，抗 CD34 抗体包被的磁性纳米颗粒（Fe3O4@PEG-CD34）显示出与干细胞的高亲和力。溶血率和部分活化凝血活酶时间（APTT）测试的结果表明，这种纳米颗粒可在血液系统中安全使用。体外研究表明，在应用的永磁体的作用下，当纳米颗粒浓度为 100μg/mL 时，细胞的保留率显著增加，对细胞活力和迁移的负面影响最小。随后的体内研究表明，纳米颗粒可以特异性地结合具有良好磁响应的干细胞。抗 CD34 抗体包被的磁性纳米颗粒可用于帮助将干细胞递送到体内的病变部位，以获得更好的治疗效果。
