Chen Jialong, Wang Shuang, Wu ZiChen, Wei Zhangao, Zhang Weibo, Li Wei
Stomatologic Hospital and College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei, Anhui 230032, China.
ACS Omega. 2019 Nov 7;4(21):19469-19477. doi: 10.1021/acsomega.9b03016. eCollection 2019 Nov 19.
Iron stents, with superior mechanical properties and controllable degradation behavior, have potential for use as feasible substitutes for nondegradable stents in the treatment of coronary artery occlusion. However, corrosion renders the iron surface hard to modify with biological molecules to accelerate endothelialization and solve restenosis. The objective of this study is to demonstrate the feasibility of using endothelial progenitor cells (EPCs) to rapidly adhere onto iron surfaces with the assistance of anti-CD34-modified magnetic nanoparticles. Transmission electron microscopy, Fourier transform infrared spectroscopy, Thermogravimetric analysis, XRD, and anti-CD34 immunofluorescence suggested that anti-CD34 and citric acid were successfully modified onto FeO, and Prussian blue staining demonstrated the selectivity of the as-prepared nanoparticles for EPCs. Under an external magnetic field (EMF), numerous nanoparticles or EPCs attached onto the surface of iron pieces, particularly the side of the iron pieces exposed to flow conditions, because iron could be magnetized under the EMF, and the magnetized iron has an edge effect. However, the uniform adhesion of EPCs on the iron stent was completed because of the weakening edge effect, and the sum of adherent EPCs was closely linked with the magnetic field (MF) intensity, which was validated by the complete covering of EPCs on the iron stent upon exposure to a 300 mT EMF within 3 h, whereas almost no cells were observed on the iron stent without an EMF. These results verify that this method can efficiently promote EPC capture and endothelialization of iron stents.
铁支架具有优异的机械性能和可控的降解行为，在冠状动脉闭塞治疗中作为不可降解支架的可行替代品具有应用潜力。然而，腐蚀使得铁表面难以用生物分子进行修饰，以加速内皮化并解决再狭窄问题。本研究的目的是证明在抗CD34修饰的磁性纳米颗粒的辅助下，内皮祖细胞（EPCs）快速粘附到铁表面的可行性。透射电子显微镜、傅里叶变换红外光谱、热重分析、XRD和抗CD34免疫荧光表明，抗CD34和柠檬酸成功修饰到FeO上，普鲁士蓝染色证明了所制备的纳米颗粒对EPCs的选择性。在外部磁场（EMF）作用下，大量纳米颗粒或EPCs附着在铁片表面，特别是铁片暴露于流动条件的一侧，因为铁在EMF下可被磁化，且磁化的铁具有边缘效应。然而，由于边缘效应减弱，EPCs在铁支架上实现了均匀粘附，且粘附的EPCs总数与磁场（MF）强度密切相关，这在3小时内暴露于300 mT EMF时铁支架上的EPCs完全覆盖得到验证，而在没有EMF的铁支架上几乎未观察到细胞。这些结果证实该方法可有效促进铁支架的EPC捕获和内皮化。
