Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4130-4135. doi: 10.1099/ijs.0.053157-0. Epub 2013 Jun 7.
A strain of Gram-staining-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacteria, designated WS-MY3(T), was isolated from a brown algae reservoir in South Korea. Strain WS-MY3(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain WS-MY3(T) fell within the cluster comprising the type strains of species of the genus Formosa, clustering coherently with the type strains of Formosa agariphila and Formosa algae. It exhibited 16S rRNA gene sequence similarity values of 98.7, 97.9 and 96.8 % to the type strains of F. agariphila, F. algae and Formosa spongicola, respectively. Strain WS-MY3(T) contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C16 : 0 3-OH, iso-C15 : 1 G and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The major polar lipids of strain WS-MY3(T) were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain WS-MY3(T) was 37.3 mol% and its DNA-DNA relatedness values with F. agariphila KCTC 12365(T) and F. algae KCTC 12364(T) were 23 % and 17 %, respectively. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WS-MY3(T) is separate from the three recognized species of the genus Formosa. On the basis of the data presented, strain WS-MY3(T) is considered to represent a novel species of the genus Formosa, for which the name Formosa undariae sp. nov. is proposed. The type strain is WS-MY3(T) ( = KCTC 32328(T) = CECT 8286(T)).
一株革兰氏染色阴性、需氧、无鞭毛、不滑行、杆状的细菌，被命名为 WS-MY3(T)，从韩国的一种褐藻水库中分离得到。WS-MY3(T) 菌株在 25°C、pH7.0-8.0 和 2.0-3.0%（w/v）NaCl 条件下生长最佳。基于 16S rRNA 基因序列的系统发育树表明，WS-MY3(T) 菌株属于 Formosa 属的种型菌株聚类群，与 Formosa agariphila 和 Formosa algae 的种型菌株聚类一致。它与 F. agariphila、F. algae 和 Formosa spongicola 的种型菌株的 16S rRNA 基因序列相似性值分别为 98.7%、97.9%和 96.8%。WS-MY3(T) 菌株含有 MK-6 作为主要的menaquinone，以及 iso-C15:0、iso-C16:0 3-OH、iso-C15:1 G 和总和特征 3（C16:1ω7c 和/或 C16:1ω6c）作为主要脂肪酸。WS-MY3(T) 菌株的主要极性脂质是磷脂酰乙醇胺和两种未鉴定的脂质。WS-MY3(T) 菌株的 DNA G+C 含量为 37.3 mol%，其与 F. agariphila KCTC 12365(T) 和 F. algae KCTC 12364(T) 的 DNA-DNA 同源性分别为 23%和 17%。系统发育和遗传上的独特性以及表型特性的差异表明，WS-MY3(T) 菌株与 Formosa 属的三个已识别种是分开的。基于所提供的数据，WS-MY3(T) 菌株被认为代表了 Formosa 属的一个新种，建议将其命名为 Formosa undariae sp. nov.。其模式菌株为 WS-MY3(T)（ = KCTC 32328(T) = CECT 8286(T)）。
