Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2013 Aug;104(2):253-60. doi: 10.1007/s10482-013-9944-y. Epub 2013 Jun 4.
A Gram-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated WS-MY6(T), was isolated from a brown algae reservoir in South Korea. Strain WS-MY6(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain WS-MY6(T) clustered with the type strains of Algibacter lectus and 'Algibacter undariae', showing 16S rRNA gene sequence similarity values of 98.1 and 98.4 %, respectively. It exhibited sequence similarities of 95.4-96.7 % to the type strains of the other Algibacter species, Pontirhabdus pectinovorans and Marinivirga aestuarii, whose reclassification into the genus Algibacter has been recently proposed. Strain WS-MY6(T) contained MK-6 as the predominant menaquinone and iso-C15:1 G, anteiso-C15:0 and iso-C17:0 3-OH as the major fatty acids. It contained phosphatidylethanolamine and two unidentified lipids as the major polar lipids. The DNA G + C content of strain WS-MY6(T) was 35.3 mol% and its DNA-DNA relatedness values with A. lectus KCTC 12103(T) and 'A. undariae' WS-MY9(T) was 21 and 13 %, respectively. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WS-MY6(T) is separate from existing Algibacter species. On the basis of the data presented, strain WS-MY6(T) is considered to represent a novel species of the genus Algibacter, for which the name Algibacter miyuki sp. nov. is proposed. The type strain is WS-MY6(T) (=KCTC 32382(T) =CECT 8300(T)).
一株革兰氏阴性、需氧、无鞭毛、不滑行的杆状细菌菌株，被命名为 WS-MY6(T)，从韩国的一种褐藻库中分离得到。菌株 WS-MY6(T) 在 25°C、pH 值 7.0-8.0 和 2%（w/v）NaCl 存在的条件下生长最佳。基于 16S rRNA 基因序列的邻接法、最大似然法和最大简约法系统发育树显示，菌株 WS-MY6(T)与 Algibacter lectus 和 'Algibacter undariae' 的模式菌株聚在一起，其 16S rRNA 基因序列相似性值分别为 98.1%和 98.4%。它与 Pontirhabdus pectinovorans 和 Marinivirga aestuarii 的模式菌株的序列相似性为 95.4-96.7%，最近有人提议将它们重新分类为 Algibacter 属。菌株 WS-MY6(T) 含有 MK-6 作为主要的menaquinone 和 iso-C15:1 G、anteiso-C15:0 和 iso-C17:0 3-OH 作为主要的脂肪酸。它含有磷脂酰乙醇胺和两种未鉴定的脂质作为主要的极性脂质。菌株 WS-MY6(T) 的 DNA G+C 含量为 35.3mol%，与 A. lectus KCTC 12103(T) 和 'A. undariae' WS-MY9(T) 的 DNA-DNA 同源性分别为 21%和 13%。系统发育和遗传上的独特性以及表型特征的差异表明，菌株 WS-MY6(T)与现有的 Algibacter 物种不同。基于所提供的数据，菌株 WS-MY6(T) 被认为代表了 Algibacter 属的一个新种，因此提议将其命名为 Algibacter miyuki sp. nov.。模式菌株为 WS-MY6(T)=KCTC 32382(T)=CECT 8300(T)。
