Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3704-3709. doi: 10.1099/ijs.0.049734-0. Epub 2013 Apr 26.
A Gram-stain-negative, non-flagellated, rod-shaped bacterial strain able to move by gliding, designated WS-MY9(T), was isolated from a brown algae reservoir in South Korea. Strain WS-MY9(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WS-MY9(T) clustered with the type strain of Algibacter lectus with a bootstrap resampling value of 100 %. Strain WS-MY9(T) exhibited 16S rRNA gene sequence similarity values of 98.5 and 96.7 % to the type strains of A. lectus and Algibacter mikhailovii, respectively, and less than 96.1 % sequence similarity to other members of the family Flavobacteriaceae. Strain WS-MY9(T) contained MK-6 as the predominant menaquinone and anteiso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 as the major fatty acids. The major polar lipids of strain WS-MY9(T) were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain WS-MY9(T) was 35.0 mol% and its DNA-DNA relatedness value with A. lectus KCTC 12103(T) was 15 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WS-MY9(T) is separate from the two recognized species of the genus Algibacter. On the basis of the data presented, strain WS-MY9(T) represents a novel species of the genus Algibacter, for which the name Algibacter undariae sp. nov. is proposed. The type strain is WS-MY9(T) ( = KCTC 32259(T) = CCUG 63684(T)).
一株能够通过滑行运动的革兰氏阴性、无鞭毛、杆状细菌菌株，被命名为 WS-MY9(T)，从韩国的一种褐藻水库中分离得到。WS-MY9(T) 菌株在 25°C、pH 值 7.0-8.0 和 2%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的邻接法系统发育树显示，WS-MY9(T) 菌株与 Algibacter lectus 模式菌株聚为一簇，自举值为 100%。WS-MY9(T) 菌株与 Algibacter lectus 和 Algibacter mikhailovii 模式菌株的 16S rRNA 基因序列相似性分别为 98.5%和 96.7%，与其他黄杆菌科成员的序列相似性小于 96.1%。WS-MY9(T) 菌株含有 MK-6 作为主要的menaquinone 和 anteiso-C15：0、iso-C17：0 3-OH、iso-C15：1 G 和 iso-C15：0 作为主要脂肪酸。WS-MY9(T) 菌株的主要极性脂为磷脂酰乙醇胺和两种未鉴定的脂类。WS-MY9(T) 菌株的 DNA G+C 含量为 35.0 mol%，其与 Algibacter lectus KCTC 12103(T) 的 DNA-DNA 同源性为 15%。系统发育和遗传的独特性以及表型特征的差异表明，WS-MY9(T) 菌株与 Algibacter 属的两个已确认种不同。基于所提供的数据，WS-MY9(T) 菌株代表了 Algibacter 属的一个新种，现提议将其命名为 Algibacter undariae sp. nov.。模式菌株为 WS-MY9(T)（=KCTC 32259(T)=CCUG 63684(T)）。
