Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4771-4776. doi: 10.1099/ijs.0.053983-0. Epub 2013 Aug 30.
A Gram-stain-negative, non-flagellated, rod-shaped bacterial strain able to move by gliding, designated WS-MY22(T), was isolated from sediment around a brown algae reservoir located on Wando in South Korea. It grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain WS-MY22(T) clustered coherently with the type strains of Algibacter lectus and Algibacter undariae. It exhibited sequence similarity of 99.4 and 98.9 % to the type strains of A. lectus and A. undariae, respectively, and of 95.1-96.6 % to those of the other species of the genus Algibacter. Strain WS-MY22(T) contained MK-6 as the predominant menaquinone and iso-C15 : 1 G and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids of strain WS-MY22(T) were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain WS-MY22(T) was 35.8 mol% and its DNA-DNA relatedness with A. lectus KCTC 12103(T) and A. undariae WS-MY9(T) was 31 and 19 %, respectively. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WS-MY22(T) is separate from other species of the genus Algibacter. On the basis of the data presented, strain WS-MY22(T) is considered to represent a novel species of the genus Algibacter, for which the name Algibacter wandonensis sp. nov. is proposed. The type strain is WS-MY22(T) ( = KCTC 32381(T) = CECT 8301(T)).
一株能够通过滑行运动的革兰氏阴性、无鞭毛、杆状细菌菌株，被命名为 WS-MY22(T)，从韩国万岛的一个褐藻水库周围的沉积物中分离得到。它在 25°C、pH7.0-8.0 和 2.0%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育树显示，菌株 WS-MY22(T)与 Algibacter lectus 和 Algibacter undariae 的模式菌株聚为一簇。它与 A. lectus 和 A. undariae 的模式菌株的序列相似性分别为 99.4%和 98.9%，与 Algibacter 属的其他种的序列相似性为 95.1-96.6%。菌株 WS-MY22(T)含有 MK-6 作为主要的menaquinone 和 iso-C15:1 G 和 iso-C17:0 3-OH 作为主要的脂肪酸。菌株 WS-MY22(T)的主要极性脂是磷脂酰乙醇胺和两种未鉴定的脂类。菌株 WS-MY22(T)的 DNA G+C 含量为 35.8 mol%，与 A. lectus KCTC 12103(T)和 A. undariae WS-MY9(T)的 DNA-DNA 同源性分别为 31%和 19%。系统发育和遗传上的独特性以及表型特征的差异表明，菌株 WS-MY22(T)与 Algibacter 属的其他种不同。基于所提供的资料，菌株 WS-MY22(T)被认为代表 Algibacter 属的一个新种，现提议将其命名为 Algibacter wandonensis sp. nov.。模式菌株为 WS-MY22(T)（ = KCTC 32381(T) = CECT 8301(T)）。
