Park Sooyeon, Park Ji-Min, Jung Yong-Taek, Kang Chul-Hyung, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
University of Science and Technology UST, 113 Gwahangno, Yuseong, Daejeon, South Korea.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2696-2701. doi: 10.1099/ijs.0.000325. Epub 2015 May 12.
A Gram-stain-negative, aerobic, non-flagellated, non-gliding and ovoid or rod-shaped bacterium, designated strain W-BA8T, was isolated from a brown algae reservoir on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain W-BA8T grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 1.0-2.0% (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain W-BA8T clustered with the type strains of species of the genus Lacinutrix. Strain W-BA8T exhibited 16S rRNA gene sequence similarity values of 94.9-96.5% to the type strains of Lacinutrix species and of less than 95.8% to the type strains of other recognized species. Strain W-BA8T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH as major fatty acids. The polar lipid profile of strain W-BA8T contained phosphatidylethanolamine, two unidentified lipids and one unidentified glycolipid as major components. The DNA G+C content of strain W-BA8T was 35 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain W-BA8T is separated from other species of the genus Lacinutrix. On the basis of the data presented, strain W-BA8T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix undariae sp. nov. is proposed. The type strain is W-BA8T ( = KCTC 42176T = CECT 8671T).
从韩国南海的一个褐藻水库中分离出一株革兰氏阴性、需氧、无鞭毛、不滑动、卵形或杆状的细菌，命名为菌株W-BA8T，并采用多相分类方法对其进行研究。菌株W-BA8T在25 °C、pH值7.0 - 7.5以及1.0 - 2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法和最大简约法系统发育树表明，菌株W-BA8T与Lacinutrix属物种的模式菌株聚类在一起。菌株W-BA8T与Lacinutrix属物种的模式菌株的16S rRNA基因序列相似性值为94.9 - 96.5%，与其他已确认物种的模式菌株的相似性值小于95.8%。菌株W-BA8T以MK-6作为主要甲基萘醌，以异-C15 : 0、异-C15 : 1 G、异-C15 : 0 3-OH和异-C17 : 0 3-OH作为主要脂肪酸。菌株W-BA8T的极性脂质谱以磷脂酰乙醇胺、两种未鉴定的脂质和一种未鉴定的糖脂为主要成分。菌株W-BA8T的DNA G+C含量为35 mol%。不同的表型特征以及系统发育独特性表明，菌株W-BA8T与Lacinutrix属的其他物种不同。根据所提供的数据，菌株W-BA8T被认为代表Lacinutrix属的一个新物种，为此提出Lacinutrix undariae sp. nov.这个名称。模式菌株为W-BA8T（ = KCTC 42176T = CECT 8671T）。
