串联富集的翻译后修饰的综合蛋白质组学分析。
Integrated proteomic analysis of post-translational modifications by serial enrichment.
机构信息
The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
出版信息
Nat Methods. 2013 Jul;10(7):634-7. doi: 10.1038/nmeth.2518. Epub 2013 Jun 9.
Abstract
We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.
摘要
我们报告了一种基于质谱的方法，通过对同一生物样品中不同翻译后修饰（SEPTM）的连续富集，实现蛋白质表达、磷酸化、泛素化和乙酰化的综合分析。这项技术使我们能够在每次实验中对近 8000 种蛋白质和超过 20000 个磷酸化、15000 个泛素化和 3000 个乙酰化位点进行定量分析，从而全面了解细胞信号转导途径，如对硼替佐米处理的人类白血病细胞的分析所示。
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