Esfahanian Fatemeh, Naimi Effat, Doroodgar Farideh, Jadali Zohre
Department of Endocrinology, Imam Khomeini Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2013 May 15;12(2):168-75.
About 25-50% of Graves' disease (GD) patients develop thyroid eye diseases, which is associated with inflammatory process and abnormalities in the levels of several cytokines in orbital tissues in GD. The aim of this study was to determine the Th1 and Th2 serum cytokines in patients with GD with or without ophthalmopathy. Serum levels of cytokines and autoantibodies including Interferon-gamma (IFN-γ), Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-10 (IL-10), TSH receptor autoantibody (TRAb), thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody(TgAb) were measured by enzyme linked immunosorbent assay(ELISA) in 34 patients with GD and in 33 normal controls. Patients were also divided in two subgroups: 18 cases with ophthalmopathy and 16 cases without ophthalmopathy. Cytokine and antibody responses were analyzed in both groups. Compared with control subjects, patients with GD showed elevated levels of IL-2 and IL-10. IFN-γ levels were lower in patients in comparison to the controls. No significant differences were found between patients and controls regarding the IL-4. There was no statistically significant difference in cytokine levels between those with or without ophthalmopathy. Quantitative-cytokine analysis demonstrated that a combination of Th1 and Th2 cytokines may contribute to the pathogenesis of GD. These results also indicate that IL-10, but not IL-4, is related to the moderate and severe forms of thyroid associated ophthalmophathy.
约25%-50%的格雷夫斯病（GD）患者会发生甲状腺眼病，这与炎症过程以及GD眼眶组织中多种细胞因子水平异常有关。本研究旨在测定患有或未患有眼病的GD患者的Th1和Th2血清细胞因子。采用酶联免疫吸附测定（ELISA）法检测了34例GD患者和33例正常对照者血清中的细胞因子和自身抗体水平，包括干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-10（IL-10）、促甲状腺激素受体自身抗体（TRAb）、甲状腺过氧化物酶抗体（TPOAb）和甲状腺球蛋白抗体（TgAb）。患者也被分为两个亚组：18例患有眼病，16例未患有眼病。对两组的细胞因子和抗体反应进行了分析。与对照受试者相比，GD患者的IL-2和IL-10水平升高。与对照组相比，患者的IFN-γ水平较低。患者与对照组在IL-4方面未发现显著差异。患有或未患有眼病的患者在细胞因子水平上无统计学显著差异。定量细胞因子分析表明，Th1和Th2细胞因子的组合可能有助于GD的发病机制。这些结果还表明，IL-10而非IL-4与中重度甲状腺相关性眼病有关。
