Department of Computation and Neural Systems, California Institute of Technology, Pasadena, California, USA.
Nat Commun. 2013;4:1965. doi: 10.1038/ncomms2965.
DNA nanotechnology has emerged as a reliable and programmable way of controlling matter at the nanoscale through the specificity of Watson-Crick base pairing, allowing both complex self-assembled structures with nanometer precision and complex reaction networks implementing digital and analog behaviors. Here we show how two well-developed frameworks, DNA tile self-assembly and DNA strand-displacement circuits, can be systematically integrated to provide programmable kinetic control of self-assembly. We demonstrate the triggered and catalytic isothermal self-assembly of DNA nanotubes over 10 μm long from precursor DNA double-crossover tiles activated by an upstream DNA catalyst network. Integrating more sophisticated control circuits and tile systems could enable precise spatial and temporal organization of dynamic molecular structures.
DNA 纳米技术已经成为一种可靠且可编程的方法，可以通过 Watson-Crick 碱基配对的特异性来控制纳米尺度上的物质，从而实现具有纳米级精度的复杂自组装结构和实现数字和模拟行为的复杂反应网络。在这里，我们展示了如何系统地整合两个成熟的框架，即 DNA 瓦片自组装和 DNA 链置换电路，以提供自组装的可编程动力学控制。我们展示了通过上游 DNA 催化剂网络激活的 DNA 双交叉瓦片前体 DNA 来触发和催化长达 10μm 以上的 DNA 纳米管的等温自组装。集成更复杂的控制电路和瓦片系统可以实现动态分子结构的精确时空组织。
