Department of Chemistry, Rutgers University, Newark, NJ, 07102, USA.
Nat Commun. 2023 Nov 23;14(1):7675. doi: 10.1038/s41467-023-43558-8.
DNA tiles serve as the fundamental building blocks for DNA self-assembled nanostructures such as DNA arrays, origami, and designer crystals. Introducing additional binding arms to DNA crossover tiles holds the promise of unlocking diverse nano-assemblies and potential applications. Here, we present one-, two-, and three-layer T-shaped crossover tiles, by integrating T junction with antiparallel crossover tiles. These tiles carry over the orthogonal binding directions from T junction and retain the rigidity from antiparallel crossover tiles, enabling the assembly of various 2D tessellations. To demonstrate the versatility of the design rules, we create 2-state reconfigurable nanorings from both single-stranded tiles and single-unit assemblies. Moreover, four sets of 4-state reconfiguration systems are constructed, showing effective transformations between ladders and/or rings with pore sizes spanning ~20 nm to ~168 nm. These DNA tiles enrich the design tools in nucleic acid nanotechnology, offering exciting opportunities for the creation of artificial dynamic DNA nanopores.
DNA 瓦片作为 DNA 自组装纳米结构的基本构建块，如 DNA 阵列、折纸和设计晶体。在 DNA 交叉瓦片上引入额外的结合臂有望解锁多样化的纳米组装和潜在应用。在这里，我们通过将 T 形结与平行交叉瓦片集成，展示了一种、两种和三种 T 形交叉瓦片。这些瓦片继承了 T 形结的正交结合方向，并保留了平行交叉瓦片的刚性，从而能够组装各种二维平铺。为了展示设计规则的多功能性，我们使用单链瓦片和单单元组件创建了 2 状态可重构纳米环。此外，还构建了四组 4 状态重构系统，展示了具有20nm 至168nm 孔径的梯状和/或环状之间的有效转换。这些 DNA 瓦片丰富了核酸纳米技术的设计工具，为创建人工动态 DNA 纳米孔提供了令人兴奋的机会。
