Center for Research at Bio/Nano Interface, Department of Chemistry, Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611 (United States), Fax: (+1) 352-846-2410; Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, (P.R. China), Fax: (+86) 23-68367257.
Chem Asian J. 2013 Oct;8(10):2417-22. doi: 10.1002/asia.201300375. Epub 2013 Jun 11.
Prostate cancer results in about 30,000 deaths annually in the United States, making it the second leading cause of cancer mortality in men in the Western world. Therefore, it is of great significance to capture and kill prostate cancer cells. It is well known that cancer stem cells are responsible for the maintenance and metastasis of tumors. This concept offers the possibility of developing a selective therapeutic approach in which cancer stem cells are directly targeted and killed. In this work, aptamers selected against DU145 prostate cancer cells (aptamer CSC1) and their subpopulation of cancer stem cells (aptamer CSC13) were linked to the surfaces of gold nanorods (AuNRs), and the resulting conjugates were successfully used to target and kill both cancer cells and cancer stem cells by near-infrared (NIR) laser irradiation. Even though cancer stem cells represent only a small population among all cancer cells, the entire cell viability was very low after laser irradiation, suggesting that tumorigenesis could be successfully controlled by this aptamer-based method, thus paving the way for early diagnosis and targeted therapy.
在美国，前列腺癌每年导致约 3 万人死亡，使其成为西方世界男性癌症死亡的第二大主要原因。因此，捕获并杀死前列腺癌细胞具有重要意义。众所周知，癌症干细胞负责肿瘤的维持和转移。这一概念为开发一种选择性治疗方法提供了可能性，即直接针对癌症干细胞并将其杀死。在这项工作中，针对 DU145 前列腺癌细胞（适体 CSC1）及其癌症干细胞亚群（适体 CSC13）的适体被连接到金纳米棒（AuNRs）的表面，所得缀合物成功地用于通过近红外（NIR）激光照射靶向和杀死癌细胞和癌症干细胞。尽管癌症干细胞仅占所有癌细胞的一小部分，但激光照射后整个细胞活力非常低，这表明这种基于适体的方法可以成功地控制肿瘤发生，从而为早期诊断和靶向治疗铺平了道路。
