Randolf H B, Haupt H, Scheibe F
HNO-Klinik der Charité, Humboldt-Universität, Berlin, German Democratic Republic.
Eur Arch Otorhinolaryngol. 1990;247(4):226-8. doi: 10.1007/BF00178990.
In order to induce acute transient local blood flow impairment in the guinea pig cochlea, terminal vessels arising from the proximal and middle third of the basilar artery and from the rostral part of the collateral vertebral artery near their junction were occluded by temporary (1-2 min) compression. Clear identification of the anterior inferior cerebellar artery was impeded due to great variation in branching of the cerebellar arteries. To quantify the effect of the vascular occlusion on the cochlear microcirculation, laser Doppler flowmetry was used. Successive compression of the cerebellar arteries resulted in individually differentiated effects on the Doppler signal from the cochlea. In about half of the experiments there was a decrease in the laser signal to 25-55% of the initial level, while in the other cases no clear decrease occurred. Proximal obstruction of the basilar artery was found to reduce the cochlear blood flow up to 10-25% of its initial level, indicating that the Doppler signal primarily reflects microcirculation within the membranous cochlea. The findings are discussed with regard to their significance as an animal model for acute transient cochlear hypoxia.
为了在豚鼠耳蜗中诱导急性短暂性局部血流受损，来自基底动脉近端和中三分之一以及椎动脉分支靠近其交界处的终末血管通过临时（1 - 2分钟）压迫进行闭塞。由于小脑动脉分支的巨大差异，难以清晰识别小脑前下动脉。为了量化血管闭塞对耳蜗微循环的影响，使用了激光多普勒血流仪。对小脑动脉的连续压迫对来自耳蜗的多普勒信号产生了个体差异的影响。在大约一半的实验中，激光信号下降到初始水平的25 - 55%，而在其他情况下没有明显下降。发现基底动脉近端阻塞可使耳蜗血流减少至其初始水平的10 - 25%，这表明多普勒信号主要反映膜迷路内的微循环。讨论了这些发现作为急性短暂性耳蜗缺氧动物模型的意义。
