Department of Electrical and Computer Engineering, Seoul National University, Seoul 151-744, Korea.
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W492-8. doi: 10.1093/nar/gkt501. Epub 2013 Jun 12.
To facilitate the analysis of large-scale high-throughput capillary electrophoresis data, we previously proposed a suite of efficient analysis software named HiTRACE (High Throughput Robust Analysis of Capillary Electrophoresis). HiTRACE has been used extensively for quantitating data from RNA and DNA structure mapping experiments, including mutate-and-map contact inference, chromatin footprinting, the Eterna RNA design project and other high-throughput applications. However, HiTRACE is based on a suite of command-line MATLAB scripts that requires nontrivial efforts to learn, use and extend. Here, we present HiTRACE-Web, an online version of HiTRACE that includes standard features previously available in the command-line version and additional features such as automated band annotation and flexible adjustment of annotations, all via a user-friendly environment. By making use of parallelization, the on-line workflow is also faster than software implementations available to most users on their local computers. Free access: http://hitrace.org.
为了便于分析大规模高通量毛细管电泳数据，我们之前提出了一套名为 HiTRACE（高通量毛细管电泳稳健分析）的高效分析软件。HiTRACE 已被广泛用于定量分析 RNA 和 DNA 结构图谱实验的数据，包括突变和图谱推断、染色质足迹、Eterna RNA 设计项目和其他高通量应用。然而，HiTRACE 基于一套命令行 MATLAB 脚本，需要花费大量精力去学习、使用和扩展。在这里，我们介绍 HiTRACE-Web，这是 HiTRACE 的在线版本，它包含了命令行版本中以前提供的标准功能，以及其他功能，如自动带注释和注释的灵活调整，所有这些都通过一个用户友好的环境。通过利用并行化，在线工作流程也比大多数用户在本地计算机上可用的软件实现更快。免费访问：http://hitrace.org。
