Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USA.
Biomed Res Int. 2013;2013:918320. doi: 10.1155/2013/918320. Epub 2013 May 13.
The human skin harbors a diverse community of bacteria, including the Gram-positive, anaerobic bacterium Propionibacterium acnes. P. acnes has historically been linked to the pathogenesis of acne vulgaris, a common skin disease affecting over 80% of all adolescents in the US. To gain insight into potential P. acnes pathogenic mechanisms, we previously sequenced the complete genome of a P. acnes strain HL096PA1 that is highly associated with acne. In this study, we compared its genome to the first published complete genome KPA171202. HL096PA1 harbors a linear plasmid, pIMPLE-HL096PA1. This is the first described P. acnes plasmid. We also observed a five-fold increase of pseudogenes in HL096PA1, several of which encode proteins in carbohydrate transport and metabolism. In addition, our analysis revealed a few island-like genomic regions that are unique to HL096PA1 and a large genomic inversion spanning the ribosomal operons. Together, these findings offer a basis for understanding P. acnes virulent properties, host adaptation mechanisms, and its potential role in acne pathogenesis at the strain level. Furthermore, the plasmid identified in HL096PA1 may potentially provide a new opportunity for P. acnes genetic manipulation and targeted therapy against specific disease-associated strains.
人体皮肤中栖息着多种细菌，包括革兰氏阳性、厌氧细菌痤疮丙酸杆菌。痤疮丙酸杆菌历来与寻常痤疮的发病机制有关，寻常痤疮是一种常见的皮肤病，影响了美国超过 80%的青少年。为了深入了解痤疮丙酸杆菌的潜在致病机制，我们之前对与痤疮高度相关的痤疮丙酸杆菌 HL096PA1 菌株进行了全基因组测序。在这项研究中，我们将其基因组与第一份公布的全基因组 KPA171202 进行了比较。HL096PA1 含有一个线性质粒，pIMPLE-HL096PA1。这是第一个被描述的痤疮丙酸杆菌质粒。我们还观察到 HL096PA1 中假基因数量增加了五倍，其中一些编码碳水化合物运输和代谢中的蛋白质。此外，我们的分析还揭示了几个仅存在于 HL096PA1 中的岛状基因组区域，以及跨越核糖体操纵子的大片段基因组倒位。这些发现为理解痤疮丙酸杆菌的毒力特性、宿主适应机制以及其在痤疮发病机制中的潜在作用提供了基础。此外，在 HL096PA1 中发现的质粒可能为痤疮丙酸杆菌的遗传操作和针对特定疾病相关菌株的靶向治疗提供新的机会。
