College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
Res Vet Sci. 2013 Oct;95(2):495-501. doi: 10.1016/j.rvsc.2013.05.006. Epub 2013 Jun 10.
The cold temperature reduces the immunity and re-production activities of the poultry. This study aimed to investigate the effects of acute and chronic cold exposure on the regulation of nuclear factor-kappa B (NF-κB) and tumor necrosis factor-α (TNF-α) expression in the duodenum, jejunum, and ileum of quails. In this study, 96 15-d-old male quails were randomly allocated into 12 groups (eight each group) for exposure to acute (up to 12h) and chronic (up to 20 days) cold stress (12 ± 1°C). Antioxidative function was examined by superoxide dismutase (SOD) and oxidative damage was examined by malondialdehyde (MDA) detection. qRT-PCR was performed to analyze expression of NF-κB and TNF-α, and DNA sequencing was performed to analyze PCR products. The data showed that under cold stress, the SOD level decreased, and the MDA level had the tendency to increase in duodenum, jejunum and ileum of quails, while the mRNA expression of NF-κB increased and TNF-α decreased in duodenum, jejunum and ileum of quails. The data from the current study indicated that both acute and chronic cold stresses were able to induce inflammatory responses in the duodenum, jejunum and ileum, which might be due to the cold-damaged intestinal oxidative stress.
低温会降低家禽的免疫力和繁殖活动。本研究旨在探讨急性和慢性冷暴露对鹌鹑十二指肠、空肠和回肠核因子-κB（NF-κB）和肿瘤坏死因子-α（TNF-α）表达调节的影响。本研究中，将 96 只 15 日龄雄性鹌鹑随机分为 12 组（每组 8 只），分别暴露于急性（最长 12 小时）和慢性（最长 20 天）冷应激（12±1°C）。通过超氧化物歧化酶（SOD）检测抗氧化功能，通过丙二醛（MDA）检测氧化损伤。采用 qRT-PCR 分析 NF-κB 和 TNF-α的表达，并对 PCR 产物进行 DNA 测序。结果显示，在冷应激下，鹌鹑十二指肠、空肠和回肠的 SOD 水平降低，MDA 水平有升高趋势，而 NF-κB 的 mRNA 表达在十二指肠、空肠和回肠中增加，TNF-α则减少。本研究数据表明，急性和慢性冷应激均能诱导鹌鹑十二指肠、空肠和回肠的炎症反应，这可能是由于冷损伤导致的肠道氧化应激。
