丝裂霉素 C 对人声带成纤维细胞的剂量依赖性作用。
Dose-dependent effect of mitomycin C on human vocal fold fibroblasts.
机构信息
Department of Hearing and Speech Sciences, University of Maryland-College Park, College Park, Maryland.
出版信息
Head Neck. 2014 Mar;36(3):401-10. doi: 10.1002/hed.23310. Epub 2013 Jun 14.
BACKGROUND
The purpose of this study was to evaluate in vitro cytotoxicity and antifibrotic effects of mitomycin C on normal and scarred human vocal fold fibroblasts.
METHODS
Fibroblasts were subjected to mitomycin C treatment at 0.2, 0.5, or 1 mg/mL, or serum control. Cytotoxicity, immunocytochemistry, and Western blot for collagen I/III were performed at days 0, 1, 3, and 5.
RESULTS
Significant decreases in live cells were measured for mitomycin C-treated cells on days 3 and 5 for all doses. Extracellular staining of collagen I/III was observed in mitomycin C-treated cells across all doses and times. Extracellular staining suggests apoptosis with necrosis, compromising the integrity of cell membranes and release of cytosolic proteins into the extracellular environment. Western blot indicates inhibition of collagen at all doses except 0.2 mg/mL at day 1.
CONCLUSION
A total of 0.2 mg/mL mitomycin C may provide initial and transient stimulation of collagen for necessary repair to damaged tissue without the long-term risk of fibrosis.
背景
本研究旨在评估丝裂霉素 C 对正常和瘢痕人声带成纤维细胞的体外细胞毒性和抗纤维化作用。
方法
将成纤维细胞用 0.2、0.5 或 1mg/ml 的丝裂霉素 C 或血清对照处理。在第 0、1、3 和 5 天进行细胞毒性、免疫细胞化学和胶原 I/III 的 Western blot 分析。
结果
在所有剂量下，第 3 和第 5 天用丝裂霉素 C 处理的细胞的活细胞数量明显减少。在所有剂量和时间下，丝裂霉素 C 处理的细胞均观察到细胞外胶原 I/III 的染色。细胞外染色提示凋亡伴有坏死，破坏细胞膜的完整性，并将细胞质蛋白释放到细胞外环境中。Western blot 表明，除了第 1 天的 0.2mg/ml 外，所有剂量均抑制胶原。
结论
0.2mg/ml 的丝裂霉素 C 可能会在不产生长期纤维化风险的情况下，为受损组织的必要修复提供初始和短暂的胶原刺激。
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