Catholic University Leuven, Department of Periodontology, Kapucijnenvoer 7, 3000 Leuven, Belgium. Catholic University Leuven, Centre for Molecular Diagnostics, Herestraat 49, 3000 Leuven, Belgium. The Weizmann Institute of Science, Biological Chemistry, Israel. The Hebrew University of Jerusalem, Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, PO Box 12, Rehovot 76100, Israel. LabMET, Laboratory of Microbial Ecology & Technology, Ghent University, B-9000 Ghent, Belgium.
Environ Microbiol Rep. 2009 Aug;1(4):228-33. doi: 10.1111/j.1758-2229.2009.00034.x. Epub 2009 May 21.
Quantification of Bdellovibrio-and-like organisms (BALOs) by microbial culturing has a number of substantial drawbacks. Therefore a quantitative PCR (qPCR) assay was designed for the culture-independent enumeration of the Bdellovibrionaceae. After optimization, the dynamic range of the qPCR assay was assessed, the specificity was evaluated and a comparison with quantitative microbial culturing was made. To evaluate the suitability of the qPCR assay for analysing environmental samples, fresh water samples were investigated by microbial culturing and by the qPCR assay. The results revealed a substantial difference between the two techniques and indicate that most Bdellovibrionaceae cells are left undetected in environmental samples when only current microbial culturing techniques are used. The application of this new technique is therefore likely to confirm the hitherto underestimated sizes and roles of predatory bacterial populations in nature.
通过微生物培养来定量 Bacteriovorax 和类似生物体 (BALO) 存在一些严重的缺陷。因此，设计了一种用于无需培养即可计数噬菌螺旋体科的定量 PCR (qPCR) 检测方法。经过优化后，评估了 qPCR 检测方法的动态范围，评估了其特异性，并与定量微生物培养进行了比较。为了评估 qPCR 检测方法分析环境样品的适用性，通过微生物培养和 qPCR 检测方法对淡水样品进行了研究。结果表明，这两种技术之间存在很大差异，表明仅当使用当前的微生物培养技术时，环境样品中大多数噬菌螺旋体科细胞未被检测到。因此，应用这项新技术可能会证实捕食性细菌群体在自然界中的大小和作用迄今被低估。
