Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Cd Reynosa, Tamaulipas 88710, Mexico.
Centro de Ciencias Genomicas, Universidad Nacional Autónoma de Mexico, Mexico.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005608.
A novel predatory bacterium, strain LBG001, has been isolated from Reynosa, Mexico. The 16S rRNA shares approximately 97 % sequence identity with many reported strains in the genus including the type strain HD100. Phylogenetic trees based on the 16S rRNA gene and on 30 concatenated housekeeping genes or core genes showed that LBG001 is on a separate branch from the group. LBG0001 has a genome size of 3 582 323 bp with a G+C content of 43.1 mol %. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values with other members of the genus (<79, <72 and <17 %, respectively) qualifies the strain to represent a new species in the genus. Strain LBG001 formed visible plaques on all 10 tested Gram-negative bacterial species. The phenotypic characteristics, phylogenetic analysis and genomic taxonomic studies support the classification of the strain as representing a new species for which the name sp. nov. is proposed. The type strain is LBG001(=ATCC TSD-288 CM-CNRG 0932).
一种新型捕食性细菌，菌株 LBG001，已从墨西哥雷诺萨分离出来。16S rRNA 与包括模式株在内的许多已报道的属内菌株的序列相似度约为 97%。基于 16S rRNA 基因和 30 个串联看家基因或核心基因的系统发育树显示，LBG001 与组内其他菌株处于不同分支。LBG0001 的基因组大小为 3,582,323 bp，G+C 含量为 43.1 mol%。与属内其他成员的平均核苷酸同一性、平均氨基酸同一性和数字 DNA-DNA 杂交值（分别<79、<72 和 <17%）表明该菌株代表该属中的一个新种。菌株 LBG001 在所有 10 种测试的革兰氏阴性细菌物种上形成可见的噬菌斑。表型特征、系统发育分析和基因组分类学研究支持将该菌株分类为代表一个新种，建议命名为 sp. nov.。模式株为 LBG001（=ATCC TSD-288 CM-CNRG 0932）。
