Herencias Cristina, Prieto Auxiliadora M, Martínez Virginia
Environmental Biology Department, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
Present address: Evolva Biotech A/S, Copenhagen, Denmark.
Bio Protoc. 2017 Mar 20;7(6):e2177. doi: 10.21769/BioProtoc.2177.
HD100 is an obligate predator that preys upon a wide variety of Gram negative bacteria. The biphasic growth cycle of includes a free-swimming attack phase and an intraperiplasmic growth phase, where the predator replicates its DNA and grows using the prey as a source of nutrients, finally dividing into individual cells (Sockett, 2009). Due to its obligatory predatory lifestyle, manipulation of requires two-member culturing techniques using selected prey microorganisms ( Lambert , 2003 ). In this protocol, we describe a detailed workflow to grow and quantify HD100 and its predatory ability, to easily carry out these laborious and time-consuming techniques.
HD100是一种专性捕食者，以多种革兰氏阴性菌为食。其双相生长周期包括自由游动的攻击阶段和周质内生长阶段，在此阶段捕食者复制其DNA并利用猎物作为营养源生长，最终分裂成单个细胞（索基特，2009年）。由于其 obligatory 捕食性生活方式，对其进行操作需要使用选定的猎物微生物的二元培养技术（兰伯特，2003年）。在本方案中，我们描述了一个详细的工作流程，用于培养和量化HD100及其捕食能力，以便轻松开展这些费力且耗时的技术。
