Ifremer, centre de Brest, Département Environnement, Microbiologie et Phycotoxines (EMP), Laboratoire de Microbiologie, ZI de la pointe du diable, B.P. 70, 29280 Plouzané, France. Ifremer, centre de Nantes, EMP/Laboratoire National de Référence Microbiologie des Coquillages, BP 21105, 44311 Nantes Cedex 3, France. Ifremer, LERPC, centre de La Rochelle, place Gaby Coll, BP 7, 17137 L'Houmeau, France.
Environ Microbiol Rep. 2010 Feb;2(1):185-91. doi: 10.1111/j.1758-2229.2010.00133.x.
The present study focused on the isolation of culturable bacteria from mussels and sea water to identify Vibrionaceae potentially pathogenic for humans. Three sites located on the French Atlantic coast were monitored monthly (twice each month during summer) for 1 year. Environmental parameters were surveyed (water temperature, salinity, turbidity, chlorophyll a) and bacteria were detected by culture and identified by API 20E(®) systems (BioMérieux) and PCR. A total of seven species were detected (Grimontia hollisae, Photobacterium damselae, Vibrio alginolyticus, V. cholerae, V. fluvialis, V. vulnificus and V. parahaemolyticus) and species diversity was higher at the end of summer. Surprisingly, V. cholerae non-O1/non-O139 was detected in spring. No site effect was detected. Using Sørensen similarity indices and statistical analyses, we showed that chlorophyll a had a significant influence on the bacterial community detected in mussels and assemblages were more similar to one another when chlorophyll a values were above 20 µg l(-1) . No significant effect of any parameter was found on the community detected in water samples. Such surveys are essential for the understanding of sanitary crises and detection of emerging pathogens.
本研究从贻贝和海水中分离可培养细菌，以鉴定可能对人类致病的弧菌类。在法国大西洋沿岸的三个地点进行了为期一年的监测，每月监测一次（夏季每月监测两次）。调查了环境参数（水温和盐度、浊度、叶绿素 a），通过培养法检测细菌，并通过 API 20E(®)系统（生物梅里埃）和 PCR 进行鉴定。共检测到七种细菌（豪氏弧菌、美人鱼发光杆菌、溶藻弧菌、霍乱弧菌、河弧菌、创伤弧菌和副溶血性弧菌），夏季末的物种多样性更高。令人惊讶的是，春季检测到非 O1/O139 霍乱弧菌。未检测到地点效应。通过使用索伦森相似性指数和统计分析，我们表明，叶绿素 a 对贻贝中检测到的细菌群落有显著影响，当叶绿素 a 值高于 20 μg l(-1) 时，群落之间的相似性更高。在水样中检测到的群落没有发现任何参数的显著影响。这种调查对于了解卫生危机和发现新出现的病原体至关重要。
