Department of Genome Oriented Bioinformatics, Technische Universität München, Wissenschaftzentrum Weihenstephan, Maximus-von-Imhof-Forum 3, D-85354 Freising, Germany.
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W486-91. doi: 10.1093/nar/gkt486. Epub 2013 Jun 12.
Although multiple biological phenomena are related to temperature (e.g. elevation of body temperature due to an illness, adaptation to environmental temperature conditions, biology of coldblooded versus warm-blooded organisms), the molecular mechanisms of these processes remain to be understood. Perturbations of secondary RNA structures may play an important role in an organism's reaction to temperature change--in all organisms from viruses and bacteria to humans. Here, we present RNAtips (temperature-induced perturbation of structure) web server, which can be used to predict regions of RNA secondary structures that are likely to undergo structural alterations prompted by temperature change. The server can also be used to: (i) detect those regions in two homologous RNA sequences that undergo different structural perturbations due to temperature change and (ii) test whether these differences are specific to the particular nucleotide substitutions distinguishing the sequences. The RNAtips web server is freely accessible without any login requirement at http://rnatips.org.
尽管许多生物学现象与温度有关（例如，由于疾病导致体温升高，适应环境温度条件，冷血生物与温血生物的生物学特性），但这些过程的分子机制仍有待理解。RNA 二级结构的改变可能在生物体对温度变化的反应中起着重要作用——从病毒和细菌到人类的所有生物体中都是如此。在这里，我们展示了 RNAtips（温度诱导的结构扰动）网络服务器，它可以用于预测 RNA 二级结构中可能发生结构改变的区域，这些改变是由温度变化引起的。该服务器还可用于：（i）检测由于温度变化导致两个同源 RNA 序列中发生不同结构改变的区域，以及（ii）检测这些差异是否是区分序列的特定核苷酸取代所特有的。RNAtips 网络服务器可在无需登录的情况下免费访问，网址为 http://rnatips.org。
