Mixed Research Unit-UMR 5623, National Center for Scientific Research-CNRS, Toulouse Institute of Chemistry, University of Toulouse, and Paul Sabatier University, Toulouse, France.
J Med Food. 2013 Jun;16(6):544-50. doi: 10.1089/jmf.2012.0275.
The chemical composition, antioxidant (DPPH and ABTS assays), anti-inflammatory (5-LOX), and cytotoxic (MCF-7) activities from flowers of seven pomegranate varieties (Punica granatum) were investigated. The highest phenolics (330.9±11.3 mg gallic acid equivalent/g dry weight (dw)), flavonoids (29.5±0.8 mg quercetin equivalent/g dw), tannins (30.6±0.6 mg catechin equivalent/g dw), and anthocyanins (0.70±0.03 mg cyanidin-3-glucoside equivalent/g dw) content were determined in the Chetoui (CH) variety. It was found that Garsi (GR) (IC₅₀=4.9±0.2 mg/L by ABTS assay) and Zaghwani (ZG) (IC₅₀=3.9±0.2 mg/L by ABTS assay) varieties exhibited the highest antioxidant activity. For the anti-inflammatory activity, all varieties were active; the ZH variety was the strongest (2.5±0.1 mg/L). The CH, ES, and RA pomegranate varieties were not active against human breast cancer cells MCF-7, whereas inhibition was more evident with extracts from ZH and GR varieties (IC₅₀=33.00±2.64 and 35.00±4.58 mg/L, respectively). Statistical analysis showed that the variety factor influenced significantly (P<.01) the chemical composition and biological activities of pomegranate flowers.
研究了七种石榴品种（Punica granatum）花的化学成分、抗氧化（DPPH 和 ABTS 测定法）、抗炎（5-LOX）和细胞毒性（MCF-7）活性。在 Chetoui（CH）品种中，测定出最高的酚类（330.9±11.3mg 没食子酸当量/g 干重（dw））、类黄酮（29.5±0.8mg 槲皮素当量/g dw）、单宁（30.6±0.6mg 儿茶素当量/g dw）和花色苷（0.70±0.03mg 矢车菊素-3-葡萄糖苷当量/g dw）含量。发现 Garsi（GR）（ABTS 测定法 IC₅₀=4.9±0.2mg/L）和 Zaghwani（ZG）（ABTS 测定法 IC₅₀=3.9±0.2mg/L）品种表现出最高的抗氧化活性。就抗炎活性而言，所有品种均具有活性；ZH 品种的活性最强（2.5±0.1mg/L）。CH、ES 和 RA 石榴品种对人乳腺癌细胞 MCF-7 没有活性，而 ZH 和 GR 品种的提取物抑制作用更为明显（IC₅₀=33.00±2.64 和 35.00±4.58mg/L）。统计分析表明，品种因素对石榴花的化学成分和生物活性有显著影响（P<.01）。
