Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China.
Chemosphere. 2013 Sep;93(2):421-7. doi: 10.1016/j.chemosphere.2013.05.022. Epub 2013 Jun 13.
Cyanobacterial blooms have received increasing attention as a public biohazard for human and animal health. To assess the effect of cyanobacteria-dominant lake water on juvenile fish, we measured the responses of specific growth rate, condition factor, body weight and body length, oxidative stress, and related gene expression of juvenile bighead carp Hypophthalmichthys nobilis exposed to in situ eutrophic lake (Chl a was around 7.0μgL(-1)). Results showed in situ cyanobacteria-dominant lake water had no effect on the growth performance, but significantly elevated the contents of malondialdehyde, the expression of heat shock protein 70, and the activity of superoxide dismutase, indicating that oxidative stress occurred. Meanwhile in situ lake water significantly decreased the expression of catalase and glutathione S-transferase genes. We conclude that in situ cyanobacteria-dominated lake water was harmful to juvenile bighead carp based on the oxidative stress and changes in the related gene expression levels.
蓝藻水华作为一种对人类和动物健康的公共生物危害受到了越来越多的关注。为了评估以蓝藻为主的湖水对幼鱼的影响，我们测量了暴露在原位富营养化湖水中（Chl a 约为 7.0μg/L）的大鳞大麻哈鱼幼鱼特定生长率、肥满度、体重和体长、氧化应激和相关基因表达的反应。结果表明，原位蓝藻为主的湖水对生长性能没有影响，但显著提高了丙二醛含量、热休克蛋白 70 的表达和超氧化物歧化酶的活性，表明发生了氧化应激。同时，原位湖水显著降低了过氧化氢酶和谷胱甘肽 S-转移酶基因的表达。我们的结论是，基于氧化应激和相关基因表达水平的变化，原位蓝藻为主的湖水对大鳞大麻哈鱼幼鱼是有害的。
