Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
Nat Methods. 2013 Aug;10(8):751-4. doi: 10.1038/nmeth.2521. Epub 2013 Jun 16.
Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs--iRFP670, iRFP682, iRFP702 and iRFP720--from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.
近红外荧光蛋白（FPs）在体内成像方面的需求很高。我们从细菌光感色素中开发了四种光谱明显不同的近红外 FPs——iRFP670、iRFP682、iRFP702 和 iRFP720。iRFPs 在哺乳动物细胞和组织中表现出高亮度，适合进行长期研究。iRFP670 和 iRFP720 可通过活细胞和小鼠中的标准方法进行双色成像。这四种新型 iRFPs 和之前设计的 iRFP713 可在活小鼠中通过光谱解混实现多色成像。
