Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA.
Nat Biotechnol. 2011 Jul 17;29(8):757-61. doi: 10.1038/nbt.1918.
Imaging biological processes in mammalian tissues will be facilitated by fluorescent probes with excitation and emission bands within the near-infrared optical window of high transparency. Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of the chromophore biliverdin and has higher effective brightness, intracellular stability and photostability than earlier phytochrome-derived fluorescent probes. Compared with far-red GFP-like proteins, iRFP has a substantially higher signal-to-background ratio in a mouse model due to its infrared-shifted spectra.
荧光探针的激发和发射波段位于近红外高透明光学窗口内，将有助于对哺乳动物组织中的生物过程进行成像。在此，我们报告了一种基于光系统 II 叶绿素 a 合酶的近红外荧光蛋白（iRFP），其激发和发射最大值分别为 690nm 和 713nm。iRFP 不需要外源性提供生色团胆绿素，并且与早期的光系统 II 衍生的荧光探针相比，具有更高的有效亮度、细胞内稳定性和光稳定性。与远红 GFP 样蛋白相比，由于 iRFP 的光谱向红外移动，因此在小鼠模型中具有更高的信号与背景比。
