Hou Yanhua, Lai Min, Chen Xiuyong, Li Jinghua, Hu Yan, Luo Zhong, Ding Xingwei, Cai Kaiyong
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China.
J Biomed Mater Res A. 2014 Jun;102(6):1726-36. doi: 10.1002/jbm.a.34839. Epub 2013 Jun 24.
To comparatively investigate the cytotoxicities of nanomaterials in circulation, in this study, three different types of nanoparticles (NPs; mesoporous SiO2, Fe3O4, and TiO2) with diameters of around 100 nm were synthesized. The morphologies, crystalline phases, and zeta potentials of those NPs were characterized by scanning electron microscopy, X-ray diffraction and zeta potential measurement, respectively. Then, we investigated the influences of different NPs on the biological functions of endothelial cells, in particular of the organelle of cells. The results indicated that different types of NPs had cytotoxic effects in a dose- and time-dependent manner, and there was no significant difference in cytotoxicity between SiO2 and Fe3O4 at concentrations <0.20 mg/mL. The shape and surface charges of NPs greatly affected cellular internalization. We found that cytoskeleton and integrity of cells were destroyed by different NPs. Additionally, the production of reactive oxygen species damaged the mitochondria of cells, in turn leading to cells apoptosis and death.
为了比较研究循环中纳米材料的细胞毒性，在本研究中，合成了三种不同类型的直径约为100nm的纳米颗粒（NPs；介孔SiO₂、Fe₃O₄和TiO₂）。分别通过扫描电子显微镜、X射线衍射和zeta电位测量对这些NPs的形态、晶相和zeta电位进行了表征。然后，我们研究了不同NPs对内皮细胞生物学功能的影响，特别是对细胞细胞器的影响。结果表明，不同类型的NPs具有剂量和时间依赖性的细胞毒性作用，在浓度<0.20mg/mL时，SiO₂和Fe₃O₄之间的细胞毒性没有显著差异。NPs的形状和表面电荷极大地影响细胞内化。我们发现不同的NPs破坏了细胞的细胞骨架和完整性。此外，活性氧的产生损害了细胞的线粒体，进而导致细胞凋亡和死亡。
