US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Food Additive Safety, Division of Food Contact Notification, College Park, MD 20740, USA.
Food Chem Toxicol. 2013 Sep;59:187-90. doi: 10.1016/j.fct.2013.06.007. Epub 2013 Jun 15.
The mouse lymphoma L5178Y Tk(+/-) assay is broadly used in toxicology to assess genotoxicity because of its known sensitivity to genotoxicants that act through a variety of mechanisms, which may include epigenetic DNA methylation. This brief article highlights the studies that have contributed to this conjecture and suggests an addition to the experimental design that could identify if the test substance is a potential epimutagen acting via hypermethylation.
鼠淋巴瘤 L5178Y TK(+/-) 检测法在毒理学中被广泛用于评估遗传毒性，因为它对通过多种机制发挥作用的遗传毒性物质具有已知的敏感性，这些机制可能包括表观遗传 DNA 甲基化。本文简要介绍了促成这一假设的研究，并提出了一种实验设计的补充方案，可以确定测试物质是否是通过过度甲基化起作用的潜在表观诱变剂。
