Department of Biological Sciences, Lehman College and the Graduate Center, City University of New York, 250 Bedford Park Boulevard West, Bronx, NY 10468, USA.
Food Chem. 2013 Nov 15;141(2):889-95. doi: 10.1016/j.foodchem.2013.03.025. Epub 2013 Mar 16.
The potential therapeutic effects of Costa Rican guava (Psidium friedrichsthalianum) extracts for chronic obstructive pulmonary disease were examined. The ethyl acetate fraction displayed the highest antioxidant activity, as compared to the hexane, chloroform, and n-butanol fractions, as well as the crude extract. This fraction was evaluated for its anti-inflammatory activity response relationship against interleukin-8 (IL-8) and inhibition of matrix metalloproteinase-1 (MMP-1) expression before and after treatment with cigarette smoke. The ethyl acetate fraction exhibited inhibitory activity against IL-8 production and MMP-1 expression, showing the most potent inhibitory activities in both assays at 100μg/mL, and nine compounds (1-9) were found. Phenolic compounds 1-O-trans-cinnamoyl-β-d-glucopyranose (2), ellagic acid (3), myricetin (4), quercitrin (7), and quercetin (9) were identified using standard compounds or literature reports from related species. Compounds 1, 5, 6, and 8 were tentatively identified as 1,5-dimethyl citrate (1), sinapic aldehyde 4-O-β-d-glucopyranose (5), 3,3',4-tri-O-methylellagic acid-4'-O-d-glucopyranoside (6), and 1,3-O-diferuloylglycerol (8), All nine compounds are reported for the first time in Costa Rican guava.
考察了哥斯达黎加番石榴（Psidium friedrichsthalianum）提取物治疗慢性阻塞性肺疾病的潜在治疗效果。与正己烷、氯仿和正丁醇部分以及粗提物相比，乙酸乙酯部分显示出最高的抗氧化活性。在评估该部分的抗炎活性时，考察了其对白细胞介素-8（IL-8）的反应关系以及对香烟烟雾处理前后基质金属蛋白酶-1（MMP-1）表达的抑制作用。乙酸乙酯部分显示出抑制 IL-8 产生和 MMP-1 表达的活性，在两种测定中，在 100μg/mL 时显示出最强的抑制活性，发现了 9 种化合物（1-9）。使用标准化合物或相关物种的文献报道鉴定了酚类化合物 1-O-反式肉桂酰基-β-d-吡喃葡萄糖苷（2）、鞣花酸（3）、杨梅素（4）、槲皮素（7）和槲皮素（9）。化合物 1、5、6 和 8 被推测为 1,5-二甲基柠檬酸酯（1）、芥子醛 4-O-β-d-吡喃葡萄糖苷（5）、3,3',4-三-O-甲基鞣花酸-4'-O-β-d-吡喃葡萄糖苷（6）和 1,3-O-二阿魏酰基甘油（8）。所有 9 种化合物均首次在哥斯达黎加番石榴中报道。
