Cuadrado-Silva Carmen Tatiana, Pozo-Bayón Maria Ángeles, Osorio Coralia
Departamento de Química, Universidad Nacional de Colombia, AA 14490 Bogotá, Colombia.
Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM), C/Nicolás Cabrera, 9, Campus de Cantoblanco, 28049 Madrid, Spain.
Molecules. 2016 Dec 23;22(1):11. doi: 10.3390/molecules22010011.
is a genus of tropical bushes belonging to the Myrtaceae family distributed in Central and South America. The polar extract of Nied. was partitioned with ethyl ether, ethyl acetate, and -butanol, and the total phenolic content and antioxidant activity were measured by Folin-Ciocalteu and ABTS assays, respectively. The ethyl acetate fraction exhibited both the highest phenolic content and antioxidant activity. Due to the complexity of this fraction, an analytical method for the comprehensive profiling of phenolic compounds was done by UPLC-ESI/QqQ in MRM (multiple reaction monitoring) mode. In this targeted analysis, 22 phenolic compounds were identified, among which several hydroxybenzoic, phenylacetic, and hydroxycinnamic acid derivatives were found. This is the first time that (+)-catechin, procyanidin B1, procyanidin B2, and (-)-epicatechin have been reported as constituents of sour guava. A fractionation by exclusion size, C-column chromatography, and preparative RRLC (rapid resolution liquid chromatography) allowed us to confirm the presence of ellagic acid and isomeric procyanidins B, well-known bioactive compounds. The content of phenolic compounds in this fruit shows its potential for the development of functional foods.
是桃金娘科的一个热带灌木属，分布于中美洲和南美洲。对Nied.的极性提取物用乙醚、乙酸乙酯和正丁醇进行分配，分别通过福林-西奥尔特（Folin-Ciocalteu）法和ABTS法测定总酚含量和抗氧化活性。乙酸乙酯部分表现出最高的酚含量和抗氧化活性。由于该部分的复杂性，采用超高效液相色谱-电喷雾电离/四极杆串联质谱（UPLC-ESI/QqQ）在多反应监测（MRM）模式下对酚类化合物进行综合分析。在该靶向分析中，鉴定出22种酚类化合物，其中发现了几种羟基苯甲酸、苯乙酸和羟基肉桂酸衍生物。这是首次报道（+）-儿茶素、原花青素B1、原花青素B2和（-）-表儿茶素是酸番石榴的成分。通过尺寸排阻、C柱色谱和制备型快速分离液相色谱（RRLC）分级分离，我们确认了鞣花酸和异构原花青素B的存在，它们是众所周知的生物活性化合物。这种水果中酚类化合物的含量显示了其在功能性食品开发方面的潜力。
