Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310027, China.
Food Chem. 2013 Nov 15;141(2):946-53. doi: 10.1016/j.foodchem.2013.04.053. Epub 2013 Apr 25.
HS002-II, a novel protein-bound polysaccharide with 44kDa molecular weight, was fractionated from submerged cultures of Hirsutella sinensis Liu, Guo, Yu & Zeng by DEAE-Sepharose and Sephacryl S200 chromatography. Based on the results of infrared spectroscopy, high performance liquid chromatography, methylation, amino acid analysis, NMR spectroscopy and atomic force microscopy, the polysaccharide moieties of HS002-II mainly contained a long backbone of (1→3)-linked α-d-ribofuranosyl units (1→4)-linked α-d-xylopyranosyl units and (1→4)-linked β-d-glucopyranosyl units, which was substituted at C-6. The two branches were β-d-mannopyranosyl residues and β-d-galactopyranosyl residues terminated with α-l-arabinopyranosyl residues, respectively. HS002-II consisted of 57.9% polysaccharide and 42.1% protein with the existence of N-type carbohydrate-protein linkage. In terms of the pro-inflammatory cytokines assay (NO, TNF-α, IL-1β and NF-κB) using murine macrophages cell line (RAW264.7), HS002-II exhibited significant immunomodulatory activity by stimulating the IκB-NF-κB pathway.
HS002-II 是一种新型的蛋白结合多糖，分子量为 44kDa，从中华被毛孢（Hirsutella sinensis）Liu、Guo、Yu 和 Zeng 的液体培养物中通过 DEAE-Sepharose 和 Sephacryl S200 层析分离得到。基于红外光谱、高效液相色谱、甲基化、氨基酸分析、NMR 光谱和原子力显微镜的结果，HS002-II 的多糖部分主要含有（1→3）-连接的α-d-核糖呋喃糖单元（1→4）-连接的α-d-木吡喃糖单元和（1→4）-连接的β-d-葡萄糖吡喃糖单元的长主链，这些单元在 C-6 位被取代。两个支链分别为β-d-甘露吡喃糖残基和β-d-半乳糖吡喃糖残基，它们的末端分别为α-l-阿拉伯吡喃糖残基。HS002-II 由 57.9%的多糖和 42.1%的蛋白质组成，存在 N 型糖蛋白连接。在使用小鼠巨噬细胞系（RAW264.7）进行促炎细胞因子测定（NO、TNF-α、IL-1β 和 NF-κB）时，HS002-II 通过刺激 IκB-NF-κB 途径表现出显著的免疫调节活性。
