SRM Research Institute, SRM University, Kattankulathur, Chennai 603203, India.
Acta Biomater. 2013 Nov;9(11):8864-74. doi: 10.1016/j.actbio.2013.06.012. Epub 2013 Jun 19.
We demonstrate a nanoparticle loading protocol to develop a transparent, multifunctional polyelectrolyte multilayer film for externally activated drug and protein delivery. The composite film was designed by alternate adsorption of poly(allylamine hydrochloride) (PAH) and dextran sulfate (DS) on a glass substrate followed by nanoparticle synthesis through a polyol reduction method. The films showed a uniform distribution of spherical silver nanoparticles with an average diameter of 50±20 nm, which increased to 80±20 nm when the AgNO3 concentration was increased from 25 to 50 mM. The porous and supramolecular structure of the polyelectrolyte multilayer film was used to immobilize ciprofloxacin hydrochloride (CH) and bovine serum albumin (BSA) within the polymeric network of the film. When exposed to external triggers such as ultrasonication and laser light the loaded films were ruptured and released the loaded BSA and CH. The release of CH is faster than that of BSA due to a higher diffusion rate. Circular dichroism measurements confirmed that there was no significant change in the conformation of released BSA in comparison with native BSA. The fabricated films showed significant antibacterial activity against the bacterial pathogen Staphylococcus aureus. Applications envisioned for such drug-loaded films include drug and vaccine delivery through the transdermal route, antimicrobial or anti-inflammatory coatings on implants and drug-releasing coatings for stents.
我们展示了一种纳米粒子加载方案，用于开发透明的多功能聚电解质多层膜，以实现外部激活的药物和蛋白质输送。该复合膜是通过在玻璃基底上交替吸附聚（盐酸烯丙胺）（PAH）和葡聚糖硫酸盐（DS），然后通过多元醇还原法合成纳米粒子来设计的。薄膜显示出具有 50±20nm 平均直径的球形银纳米粒子的均匀分布，当 AgNO3 浓度从 25mM 增加到 50mM 时，直径增加到 80±20nm。聚电解质多层膜的多孔和超分子结构用于将盐酸环丙沙星（CH）和牛血清白蛋白（BSA）固定在膜的聚合物网络内。当暴露于外部触发因素（如超声和激光）时，负载的薄膜破裂并释放负载的 BSA 和 CH。由于扩散速率较高，CH 的释放速度快于 BSA。圆二色性测量证实，与天然 BSA 相比，释放的 BSA 的构象没有明显变化。所制备的薄膜对细菌病原体金黄色葡萄球菌表现出显著的抗菌活性。这种载药薄膜的应用设想包括通过透皮途径输送药物和疫苗、在植入物上涂抗菌或抗炎涂层以及在支架上涂释药涂层。
