Jin Guorui, Prabhakaran Molamma P, Kai Dan, Ramakrishna Seeram
Department of Mechanical Engineering, National University of Singapore, Singapore, Singapore; Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):689-98. doi: 10.1016/j.ejpb.2013.06.002. Epub 2013 Jun 18.
With advances in the field of tissue engineering, it is increasingly recognized that biodegradable and biocompatible scaffolds incorporated with multiple wound healing mediators might serve as the most promising medical devices for skin tissue regeneration. Through controlled drug delivery, these medical devices can reduce the toxicity effects and optimize clinical efficiency. In this study, we first encapsulated multiple epidermal induction factors (EIF) such as the epidermal growth factor (EGF), insulin, hydrocortisone, and retinoic acid (RA) with gelatin and poly(L-lactic acid)-co-poly-(ε-caprolactone) (PLLCL) solutions and performed electrospinning by two different approaches: blend spinning and core-shell spinning. No burst release was detected from EIF encapsulated core-shell nanofibers; however, an initial 44.9% burst release from EIF blended nanofibers was observed over a period of 15 days. The epidermal differentiation potential of adipose-derived stem cells (ADSCs) was evaluated for EIF-containing scaffolds prepared either by core-shell spinning or by blend spinning. After 15 days of cell culture, the proliferation of ADSCs on EIF encapsulated core-shell nanofibers was the highest. Moreover, a higher percentage of ADSCs got differentiated to epidermal lineages on EIF encapsulated core-shell nanofibers compared to the cell differentiation on EIF blended nanofibers, which can be attributed to the sustained release of EIF from the core-shell nanofibers. Our study demonstrated that the EIF encapsulated core-shell nanofibers might serve as a promising tissue engineered graft for skin regeneration.
随着组织工程领域的进展，人们越来越认识到，结合多种伤口愈合介质的可生物降解且生物相容的支架可能成为皮肤组织再生最有前景的医疗设备。通过控制药物释放，这些医疗设备可以降低毒性作用并优化临床疗效。在本研究中，我们首先用明胶和聚（L-乳酸）-共-聚（ε-己内酯）（PLLCL）溶液包裹多种表皮诱导因子（EIF），如表皮生长因子（EGF）、胰岛素、氢化可的松和视黄酸（RA），并通过两种不同方法进行静电纺丝：共混纺丝和核壳纺丝。从EIF包裹的核壳纳米纤维中未检测到突释现象；然而，在15天的时间里，观察到EIF共混纳米纤维有44.9%的初始突释。对通过核壳纺丝或共混纺丝制备的含EIF支架评估脂肪干细胞（ADSC）的表皮分化潜能。细胞培养15天后，ADSC在EIF包裹的核壳纳米纤维上的增殖最高。此外，与EIF共混纳米纤维上的细胞分化相比，EIF包裹的核壳纳米纤维上有更高比例的ADSC分化为表皮谱系，这可归因于EIF从核壳纳米纤维中的持续释放。我们的研究表明，EIF包裹的核壳纳米纤维可能成为用于皮肤再生的有前景的组织工程移植物。
