Diabetes, Obesity and Human Reproduction Group, Hospital Universitario Ramón y Cajal, Universidad de Alcalá, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas CIBERDEM, Madrid, Spain.
Mol Cell Endocrinol. 2013 Aug 25;376(1-2):99-106. doi: 10.1016/j.mce.2013.06.010. Epub 2013 Jun 18.
The mechanisms involved in the progression to overt diabetes in pre-obese subjects remain unclear. Therefore, a nontargeted evaluation of differences in the protein abundance of visceral adipose tissue (VAT) obtained from pre-obese diabetic subjects and pre-obese subjects showing normal glucose tolerance may provide novel insights on the molecular processes involved in the progression to overt diabetes in pre-obesity. Diabetic patients showed increased VAT abundance of glutathione S-transferase Mu 2, peroxiredoxin-2, antithrombin-III, apolipoprotein A-IV, Ig κ chain C region, mitochondrial aldehyde dehydrogenase and actin, and decreased abundance of annexin-A1, retinaldehyde dehydrogenase-1, and vinculin, compared with their non-diabetic counterparts. These proteins are involved in cytoskeleton function and structure, oxidative stress, inflammation and retinoid metabolism. The presence of diabetes influences the VAT abundance of several proteins. Hence, the proteins identified here could be considered candidate molecules in future studies addressing the role that VAT dysfunction plays in the development of type 2 diabetes.
在肥胖前期人群中，向显性糖尿病进展的相关机制尚不清楚。因此，对肥胖前期糖尿病患者和糖耐量正常的肥胖前期患者内脏脂肪组织（VAT）中蛋白质丰度的非靶向评估，可能为肥胖前期向显性糖尿病进展的分子过程提供新的见解。与非糖尿病患者相比，糖尿病患者的 VAT 中谷胱甘肽 S-转移酶 Mu2、过氧化物酶-2、抗凝血酶-III、载脂蛋白 A-IV、免疫球蛋白 κ 链 C 区、线粒体醛脱氢酶和肌动蛋白的丰度增加，而膜联蛋白-A1、视黄醛脱氢酶-1 和纽蛋白的丰度降低。这些蛋白质参与细胞骨架功能和结构、氧化应激、炎症和类视黄醇代谢。糖尿病的存在影响了 VAT 中几种蛋白质的丰度。因此，这里鉴定到的蛋白质可以被认为是未来研究中候选的分子，这些研究旨在探讨 VAT 功能障碍在 2 型糖尿病发展中的作用。
