Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
Infect Genet Evol. 2013 Oct;19:71-80. doi: 10.1016/j.meegid.2013.06.013. Epub 2013 Jun 19.
Genotype G4P[6] Rotavirus A (RVA) strains collected from children admitted to hospital with gastroenteritis over a 15 year period in the pre rotavirus vaccine era in Hungary were characterized in this study. Whole genome sequencing and phylogenetic analysis was performed on eight G4P[6] RVA strains. All these RVA strains shared a fairly conservative genomic configuration (G4-P[6]-I1/I5-R1-C1-M1-A1/A8-N1-T1/T7-E1-H1) and showed striking similarities to porcine and porcine-derived human RVA strains collected worldwide, although genetic relatedness to some common human RVA strains was also seen. The resolution of phylogenetic relationship between porcine and human RVA genes was occasionally low, making the evaluation of host species origin of individual genes sometimes difficult. Yet the whole genome constellations and overall phylogenetic analyses indicated that these eight Hungarian G4P[6] RVA strains may have originated by independent zoonotic transmission, probably from pigs. Future surveillance studies of human and animal RVA should go parallel to enable the distinction between direct interspecies transmission events and those that are coupled with reassortment of cognate genes.
本研究对在轮状病毒疫苗问世前的 15 年间，匈牙利因肠胃炎住院的儿童中采集到的基因型为 G4P[6]的轮状病毒 A（RVA）株进行了特征描述。对 8 株 G4P[6] RVA 株进行了全基因组测序和系统进化分析。所有这些 RVA 株具有相当保守的基因组结构（G4-P[6]-I1/I5-R1-C1-M1-A1/A8-N1-T1/T7-E1-H1），与全球范围内采集的猪源性和猪源人源性 RVA 株非常相似，尽管与一些常见的人源性 RVA 株也存在遗传相关性。猪源性和人源性 RVA 基因的系统进化关系分辨率偶尔较低，使得评估个别基因的宿主物种起源有时较为困难。然而，全基因组结构和整体系统进化分析表明，这 8 株匈牙利 G4P[6] RVA 株可能通过独立的人畜共患病传播而产生，可能源自猪。未来对人和动物 RVA 的监测研究应同时进行，以便区分直接的种间传播事件和那些与同源基因重组相关的事件。
