Itoh Y, Rader M D, Reichlin M
University of Oklahoma Health Sciences Center, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Clin Exp Immunol. 1990 Jul;81(1):45-51. doi: 10.1111/j.1365-2249.1990.tb05289.x.
Precipitating antibodies to the Ro/SSA antigen occur in the sera of 40% of patients with systemic lupus erythematosus (SLE) and in 40-70% of the sera of patients with primary Sjögren's syndrome. Previous work has shown that lymphocyte extracts contain two Ro/SSA antigens with protein moieties of 60 kD and 52 kD and that erythrocyte haemolysate contain two analogous but antigenically distinct Ro/SSA molecules of 60 kD and 54 kD. Frequency analysis of the various specificities in 43 sera with precipitating anti-Ro/SSA and studies with affinity-eluted antibodies suggest that the lymphocyte 60 kD and erythrocyte 60 kD Ro/SSA molecules are related as are the lymphocyte 52 kD and erythrocyte 54 kD Ro/SSA proteins. Anti-Ro/SSA sera when accompanied by other precipitins (anti-La/SSB and anti-U1RNP) react preferentially with certain Ro/SSA isoforms. Evidence is also presented for a 45 kD form of Ro/SSA. These data suggest that the antigenic heterogeneity of the Ro/SSA antigen is immunologically relevant and that there are two families of Ro/SSA antigens; one comprising of the two 60-kD proteins in the erythrocyte and lymphocyte and the other the erythrocyte 54 kD and lymphocyte 52 kD Ro/SSA proteins, respectively.
针对Ro/SSA抗原的沉淀抗体出现在40%的系统性红斑狼疮（SLE）患者血清中，以及40%-70%的原发性干燥综合征患者血清中。先前的研究表明，淋巴细胞提取物含有两种Ro/SSA抗原，其蛋白质部分分别为60kD和52kD，红细胞溶血产物含有两种类似但抗原性不同的Ro/SSA分子，分别为60kD和54kD。对43份含有抗Ro/SSA沉淀抗体的血清中各种特异性的频率分析以及对亲和洗脱抗体的研究表明，淋巴细胞60kD和红细胞60kD的Ro/SSA分子相关，淋巴细胞52kD和红细胞54kD的Ro/SSA蛋白也相关。当抗Ro/SSA血清伴有其他沉淀素（抗La/SSB和抗U1RNP）时，会优先与某些Ro/SSA亚型发生反应。还发现了一种45kD形式的Ro/SSA。这些数据表明，Ro/SSA抗原的抗原异质性具有免疫学相关性，并且存在两个Ro/SSA抗原家族；一个家族分别由红细胞和淋巴细胞中的两种60kD蛋白质组成，另一个家族分别由红细胞54kD和淋巴细胞52kD的Ro/SSA蛋白组成。
