Deguchi Y, Shibata N, Kishimoto S
Third Department of Internal Medicine, Osaka University School of Medicine, Japan.
Clin Exp Immunol. 1990 Jul;81(1):97-100. doi: 10.1111/j.1365-2249.1990.tb05297.x.
Scleroderma is a systemic disorder characterized by fibrosis, which affects skin, lung, kidney and other organs. Heat shock protein (hsp) (70 kD) has been implicated as an essential element of cell function in cell growth and differentiation. To study the molecular basis of intracellular events in scleroderma fibroblasts, we compared the expression of hsp 70 gene in scleroderma and normal control fibroblasts by nuclear run on transcription assay and Northern blot assay. We show that scleroderma fibroblasts express more than eight times higher level of hsp 70 transcription in normal control fibroblasts at quiescent conditions in the absence of serum. After stimulation with serum, the transcription level of the hsp 70 gene is similar in scleroderma and normal control fibroblasts. Therefore, our results indicate an alteration/activation of intracellular events in scleroderma fibroblasts.
硬皮病是一种以纤维化为特征的全身性疾病，可影响皮肤、肺、肾和其他器官。热休克蛋白（hsp）（70 kD）被认为是细胞生长和分化中细胞功能的重要元素。为了研究硬皮病成纤维细胞内事件的分子基础，我们通过核转录分析和Northern印迹分析比较了硬皮病和正常对照成纤维细胞中hsp 70基因的表达。我们发现，在无血清的静止条件下，硬皮病成纤维细胞中hsp 70转录水平比正常对照成纤维细胞高八倍以上。血清刺激后，硬皮病和正常对照成纤维细胞中hsp 70基因的转录水平相似。因此，我们的结果表明硬皮病成纤维细胞内事件发生了改变/激活。
