Anal Chem. 2013 Jul 16;85(14):6571-4. doi: 10.1021/ac4014594. Epub 2013 Jun 26.
We developed a colorimetric sensor array with reported protein aptamers as nonspecific receptors. We found that different target proteins could make the aptamer-protected gold nanoparticles (AuNPs) exhibit different aggregation behaviors in the presence of a high concentration salt and cause various color change. On the basis of this phenomenon, we applied a series of reported protein aptamers as a receptor array obtaining a distinct response pattern to each target protein. Seven proteins have been well distinguished with the naked eye at the 50 nM level. Cancerous human cells have also been discriminated from noncancerous cells. This method is simple, label-free, and sensitive. It will broaden the application filed of plasmonic nanoparticle-based sensors and give a new direction of developing sensitive array sensing systems.
我们开发了一种比色传感器阵列，其中报道的蛋白质适体作为非特异性受体。我们发现不同的靶蛋白在高浓度盐存在下可以使适体保护的金纳米颗粒（AuNPs）表现出不同的聚集行为，并导致各种颜色变化。基于这一现象，我们应用了一系列报道的蛋白质适体作为受体阵列，对每种靶蛋白获得了独特的响应模式。在 50 nM 水平下，七种蛋白质已经可以用肉眼很好地区分。还可以区分癌细胞和非癌细胞。该方法简单、无标记且灵敏。它将拓宽基于等离子体纳米粒子的传感器的应用领域，并为开发灵敏的阵列传感系统提供新的方向。
