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一个来自水稻的 MAPKKK 基因，RBG1res，通过负调控 ABA 赋予对伯克霍尔德氏菌的抗性。

A MAPKKK gene from rice, RBG1res, confers resistance to Burkholderia glumae through negative regulation of ABA.

机构信息

Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan.

Strategic Planning Headquarters, NARO, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8517, Japan.

出版信息

Sci Rep. 2023 Mar 9;13(1):3947. doi: 10.1038/s41598-023-30471-9.

DOI:10.1038/s41598-023-30471-9
PMID:36894555
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9998638/
Abstract

Burkholderia glumae causes bacterial seedling rot (BSR) of rice and is a threat to a consistent food supply. When previously screening for resistance against B. glumae in the resistant cultivar Nona Bokra (NB) versus the susceptible cultivar Koshihikari (KO), we detected a gene, Resistance to Burkholderia glumae 1 (RBG1), at a quantitative trait locus (QTL). Here, we found that RBG1 encodes a MAPKKK gene whose product phosphorylates OsMKK3. We also found that the kinase encoded by the RBG1 resistant (RBG1res) allele in NB presented higher activity than did that encoded by the RBG1 susceptible (RBG1sus) allele in KO. RBG1res and RBG1sus differ by three single-nucleotide polymorphisms (SNPs), and the G390T substitution is essential for kinase activity. Abscisic acid (ABA) treatment of inoculated seedlings of RBG1res-NIL (a near-isogenic line (NIL) expressing RBG1res in the KO genetic background) decreased BSR resistance, indicating that RBG1res conferred resistance to B. glumae through negative regulation of ABA. The results of further inoculation assays showed that RBG1res-NIL was also resistant to Burkholderia plantarii. Our findings suggest that RBG1res contributes to resistance to these bacterial pathogens at the seed germination stage via a unique mechanism.

摘要

腐皮镰刀菌引起水稻细菌性苗腐病（BSR），是粮食供应的威胁。在先前筛选抗腐皮镰刀菌的过程中，我们在抗性品种 Nona Bokra（NB）与感病品种 Koshihikari（KO）中检测到一个数量性状位点（QTL）上的基因 Resistance to Burkholderia glumae 1（RBG1）。这里，我们发现 RBG1 编码一个 MAPKKK 基因，其产物可磷酸化 OsMKK3。我们还发现，NB 中 RBG1 抗性（RBG1res）等位基因编码的激酶比 KO 中 RBG1 敏感（RBG1sus）等位基因编码的激酶活性更高。RBG1res 和 RBG1sus 之间存在三个单核苷酸多态性（SNPs），而 G390T 取代对激酶活性是必需的。ABA 处理 RBG1res-NIL（在 KO 遗传背景中表达 RBG1res 的近等基因系（NIL））接种幼苗后，BSR 抗性降低，表明 RBG1res 通过负调控 ABA 赋予对 B. glumae 的抗性。进一步接种试验的结果表明，RBG1res-NIL 对 Burkholderia plantarii 也具有抗性。我们的研究结果表明，RBG1res 通过独特的机制在种子萌发阶段有助于对这些细菌病原体的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/e21a7b2150c6/41598_2023_30471_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/f7b70eaff71c/41598_2023_30471_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/df6d49133f21/41598_2023_30471_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/e8b65bf33530/41598_2023_30471_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/6e80372e55ef/41598_2023_30471_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/18f37e80b5e3/41598_2023_30471_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/e21a7b2150c6/41598_2023_30471_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/f7b70eaff71c/41598_2023_30471_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/df6d49133f21/41598_2023_30471_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/e8b65bf33530/41598_2023_30471_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/6e80372e55ef/41598_2023_30471_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/18f37e80b5e3/41598_2023_30471_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c3/9998638/e21a7b2150c6/41598_2023_30471_Fig6_HTML.jpg

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