Sadeghi-Aliabadi Hojjat, Mozaffari Morteza, Behdadfar Behshid, Raesizadeh Maryam, Zarkesh-Esfahani Hamid
School of Pharmacy, Isfahan Pharmaceutical Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Avicenna J Med Biotechnol. 2013 Apr;5(2):96-103.
Magnetic nanoparticles in a variable magnetic field are able to produce heat. This heat (42-45°C) has more selective effect on fast dividing cancer cells than normal tissues.
In this work magnetite nanoparticles have been prepared via co-precipitation and phase identification was performed by powder x-ray diffraction (XRD). Magnetic parameters of the prepared nanoparticles were measured by a Vibrating Sample Magnetometer (VSM). A sensitive thermometer has been used to measure the increase of temperature in the presence of an alternating magnetic field. To evaluate the cytotoxicity of nanoparticles, the suspended magnetite nanoparticles in liquid paraffin, doxorubicin and a mixture of both were added to the MDA-MB-468 cells in separate 15 ml tubes and left either in the RT or in the magnetic field for 30 min. Cell survival was measured by trypan blue exclusion assay and flow cytometer. Particle size distribution of the nanoparticles was homogeneous with a mean particles size of 10 nm. A 15°C temperature increase was achieved in presence of an AC magnetic field after 15 min irradiation.
Biological results showed that magnetite nanoparticles alone were not cytotoxic at RT, while in the alternative magnetic filed more than 50% of cells were dead. Doxorubicin alone was not cytotoxic during 30 min, but in combination with magnetite more than 80% of the cells were killed.
It could be concluded that doxorubicin and magnetite nanoparticles in an AC magnetic field had combinatory effects against cells.
磁性纳米颗粒在可变磁场中能够产热。这种热量（42 - 45°C）对快速分裂的癌细胞的选择性作用比对正常组织更强。
在本研究中，通过共沉淀法制备了磁铁矿纳米颗粒，并通过粉末X射线衍射（XRD）进行相鉴定。用振动样品磁强计（VSM）测量所制备纳米颗粒的磁性参数。使用灵敏温度计测量交变磁场存在时的温度升高。为了评估纳米颗粒的细胞毒性，将悬浮在液体石蜡中的磁铁矿纳米颗粒、阿霉素以及二者的混合物分别加入到15 ml试管中的MDA - MB - 468细胞中，然后置于室温或磁场中30分钟。通过台盼蓝排斥试验和流式细胞仪测量细胞存活率。纳米颗粒的粒径分布均匀，平均粒径为10 nm。照射15分钟后，在交变磁场存在的情况下温度升高了15°C。
生物学结果表明，室温下单独的磁铁矿纳米颗粒没有细胞毒性，而在交变磁场中超过50%的细胞死亡。单独的阿霉素在30分钟内没有细胞毒性，但与磁铁矿联合使用时超过80%的细胞被杀死。
可以得出结论，阿霉素和磁铁矿纳米颗粒在交变磁场中对细胞具有联合作用。
