Park Hyun, Ka Kang-Hyeon, Ryu Sung-Ryul
Korea Forest Research Institute, Seoul 130-712, Korea.
Korea Forest Seed & Variety Center, Chungju 380-941, Korea.
Mycobiology. 2014 Mar;42(1):41-5. doi: 10.5941/MYCO.2014.42.1.41. Epub 2014 Mar 31.
The effectiveness of three kinds of enzymes (chitinase, β-glucuronidase, and lysing enzyme complex), employed as elicitors to enhance the β-glucan content in the sawdust-based cultivation of cauliflower mushroom (Sparassis latifolia), was examined. The elicitors were applied to the cauliflower mushroom after primordium formation, by spraying the enzyme solutions at three different levels on the sawdust-based medium. Mycelial growth was fully accomplished by the treatments, but the metabolic process during the growth of fruiting bodies was affected. The application of a lysing enzyme resulted in an increase in the β-glucan concentration by up to 31% compared to that of the control. However, the treatment resulted in a decrease in mushroom yield, which necessitated the need to evaluate its economic efficiency. Although we still need to develop a more efficient way for using elicitors to enhance functional metabolites in mushroom cultivation, the results indicate that the elicitation technique can be applied in the cultivation of medicinal/edible mushrooms.
研究了三种酶（几丁质酶、β-葡萄糖醛酸酶和溶菌酶复合物）作为诱导剂在木屑栽培花椰菜菇（Sparassis latifolia）中提高β-葡聚糖含量的有效性。在原基形成后，通过在木屑培养基上以三种不同水平喷洒酶溶液，将诱导剂应用于花椰菜菇。处理完成了菌丝体的生长，但子实体生长过程中的代谢过程受到了影响。与对照相比，溶菌酶的应用使β-葡聚糖浓度提高了31%。然而，该处理导致蘑菇产量下降，因此有必要评估其经济效益。尽管我们仍需开发一种更有效的方法来利用诱导剂提高蘑菇栽培中的功能代谢产物，但结果表明诱导技术可应用于药用/食用蘑菇的栽培。
